Anti-HDLBP Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q00341

Core Product Specifications and Parameters

Biological Background: Vigilin Function and Localization

• Vigilin, also known as HDL-binding protein (HDLBP), is encoded by the HDLBP gene in humans (Gene ID: 3069). Alternative splicing generates multiple isoforms.
• It is an RNA-binding protein that contains multiple KH domains, which are characteristic repeats known to mediate RNA interactions.
• Vigilin is predominantly localized in the cytoplasm and nucleus, reflecting its involvement in both cytoplasmic and nuclear processes.
• Functionally, Vigilin appears to play a role in cellular sterol metabolism, potentially protecting cells from excessive cholesterol accumulation.
• It is subject to post-translational modifications, including acetylation and phosphorylation, which may regulate its activity and interactions.
• Vigilin has been implicated in high-density lipoprotein (HDL) binding and lipid transport, linking it to cardiovascular and metabolic disease research.

Experimental Guidance and Technical Tips

• The immunogen consists of amino acids 1035–1268 of human HDLBP. For Western blotting, consider using whole-cell lysates from human, mouse, or rat tissues or cell lines; the expected band is at approximately 141 kDa.
• For IHC-P, validate the antibody on the relevant formalin-fixed paraffin-embedded tissue sections and optimize antigen retrieval conditions.
• In IF/ICC, standard fixation and permeabilization protocols should be tested; appropriate controls are recommended to confirm specificity.
• ELISA procedures should follow standard indirect or sandwich ELISA protocols with the antibody used as the detection or capture reagent, as appropriate.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories by providing a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our HDLBP/Vigilin antibody supports investigations into cholesterol metabolism, lipoprotein biology, and related disorders. Whether you require rare-target reagents, technical consultation, or scalable supply, CamelBio bridges the gap from concept to clinic, helping you accelerate assay development with confidence.