Anti-HDGF Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P51858

Core Product Specifications and Parameters

Biological Background: HDGF Function and Localization

• HDGF (hepatoma-derived growth factor), also known as HMG1L2, is a multifunctional protein with transcriptional repressor activity. Related references: PMID:17974029
• It exhibits mitogenic activity for fibroblasts, promoting cell proliferation. Related references: PMID:11751870, PMID:26845719
• HDGF is a heparin-binding protein, which may contribute to its extracellular functions. Related references: PMID:15491618
• Alternative splicing generates isoforms that lack mitogenic activity and heparin-binding capacity. Related references: PMID:26845719
• The protein localizes to the nucleus, cytoplasm, and is secreted; it is also found in extracellular exosomes.
• Ubiquitously expressed across tissues, reflecting its broad regulatory roles.
• Post-translational modifications include acetylation, phosphorylation, and isopeptide bond formation; it is also subject to ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length human HDGF (aa 1–240), making this antibody suitable for detecting multiple isoforms. Validate specificity in your sample system, especially when working with tissues or cell lines expressing alternative isoforms.
• For Western blot (WB), the expected molecular weight is approximately 27 kDa. Optimize loading controls and antibody dilution to achieve clear bands.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), consider appropriate antigen retrieval and permeabilization protocols, respectively. Test dilution ranges and include known positive and negative controls.
• Cross-reactivity with mouse and rat HDGF has been demonstrated, enabling studies across these species. Confirm cross-reactivity in your experimental context if working with other organisms.
• As a polyclonal antibody, lot-to-lot variability may occur. Include internal reference samples and consider re-validating critical applications with each new batch.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies like this anti-HDGF antibody, and bulk ancillary reagents. With expertise in rare target sourcing and a commitment to supporting projects from concept to clinic, CamelBio empowers your HDGF-related research and assay development with reliable, high-quality reagents and technical consultation.