Anti-HDGF Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P51858

Core Product Specifications and Parameters

Biological Background: HDGF Function and Localization

• HDGF (Hepatoma-derived growth factor), also known as HMG1L2, is a member of the high mobility group protein family and functions as a transcriptional repressor.
  Related references: PMID:17974029
• It can act as a mitogenic growth factor for fibroblasts, though this activity varies among isoforms.
  Related references: PMID:11751870, PMID:26845719
• HDGF is a heparin-binding protein, with some isoforms lacking this property.
  Related references: PMID:15491618, PMID:26845719
• Subcellular localization includes nucleus, cytoplasm, and secretion into extracellular space, including exosomes.
• It is ubiquitously expressed across tissues and undergoes post-translational modifications such as acetylation, phosphorylation, and ubiquitination, and is involved in DNA binding and transcriptional regulation.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1-240 of human HDGF, suggesting that the antibody may recognize multiple isoforms.
• For Western blot, expect a band around 27kDa; consider using HeLa or HepG2 cell lysates as positive controls.
• For immunohistochemistry (IHC-P), antigen retrieval may be required; test citrate or EDTA buffer at pH 6.0 or 9.0.
• In immunofluorescence (IF/ICC), optimize fixation (e.g., 4% PFA) and permeabilization conditions for the specific cell type.
• When using ELISA, pair with a matched detection antibody or capture molecule for best sensitivity.
• Always validate the antibody in your specific sample system and application before routine use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a streamlined one-stop supply of IVD raw materials. This HDGF antibody supports research in transcriptional regulation, growth factor signaling, and heparin-binding pathways, and we can source validated antibody pairs, optimized monoclonals/polyclonals, bulk ancillary reagents, and rare-target raw materials to accelerate your assay development. Let CamelBio’s expertise bridge your concept to clinic.