Anti-HDC Rabbit Polyclonal Antibody for WB, ELISA - P19113

Core Product Specifications and Parameters

Biological Background: Histidine decarboxylase Function and Localization

• Histidine decarboxylase (HDC) catalyzes the conversion of L-histidine to histamine, a key step in histamine biosynthesis.
• It is a member of the group II decarboxylase family and utilizes pyridoxal phosphate (PLP) as a cofactor.
• The enzyme plays a role in catecholamine biosynthesis, linking histamine production to broader amine metabolism.
• Alternative splicing of HDC generates multiple transcript variants, potentially producing different protein isoforms.
• HDC has been identified in proteomics studies, indicating its expression in various tissues and conditions.
• Its 3D structure has been solved, providing insights into its catalytic mechanism and regulatory properties.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 453-662) of human HDC, which may target functional epitopes and minimize interference with active site residues.
• For Western blot, the expected protein molecular weight is approximately 74 kDa; validate band specificity in relevant human or mouse cell lysates expressing HDC.
• In ELISA applications, the polyclonal antibody may offer sensitive detection due to recognition of multiple epitopes; consider optimizing coating and blocking conditions.
• Given cross-reactivity with mouse, this antibody could be suitable for comparative studies between human and mouse samples; verify performance in each experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw-material sourcing, offering a comprehensive portfolio of validated antibodies, reagents, and technical support across the diagnostic development pipeline. This anti-HDC rabbit polyclonal antibody supports research into histamine biosynthesis, neuroendocrine function, and inflammatory pathways. Beyond individual antibodies, we provide matched antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets to accelerate your assay development from concept to clinic.