Anti-HDC Polyclonal Antibody for WB, ELISA - P19113

Core Product Specifications and Parameters

Biological Background: Histidine Decarboxylase Function and Localization

• Histidine decarboxylase (HDC) catalyzes the biosynthesis of histamine by decarboxylating the amino acid histidine.
• The enzyme requires pyridoxal phosphate (PLP) as a cofactor for its catalytic activity.
• Alternative splicing of the HDC gene produces multiple transcript variants encoding different protein isoforms.
• The canonical HDC protein has a predicted molecular weight of approximately 74 kDa.
• HDC is classified under lyase and decarboxylase enzyme families.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 2–249 of human HDC; consider that the resulting polyclonal antibody may detect multiple epitopes across this N-terminal region.
• For Western blot (WB), bands near 70–74 kDa are expected; validate the molecular weight in your sample system and include appropriate controls.
• The antibody is specified for WB and ELISA; for ELISA applications, optimize coating and detection conditions using a relevant histidine decarboxylase standard.
• Cross-reactivity with mouse and rat HDC is reported; verify sequence homology within the immunogen region to ensure detection in these species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined procurement channel for IVD manufacturers and research laboratories seeking high-quality raw materials. This anti-HDC polyclonal antibody, targeting a key enzyme in histamine biosynthesis, is available alongside a broad portfolio of antibodies, antigens, and ancillary reagents. Our team supports clients from discovery to validation with customized sourcing solutions and technical consultation.