Anti-HDAC9 Rabbit Polyclonal Antibody for WB - Q9UKV0

Core Product Specifications and Parameters

Biological Background: HDAC9 Function and Localization

• HDAC9 is a histone deacetylase that catalyzes removal of acetyl groups from lysine residues on N-terminal tails of core histones (H2A, H2B, H3, H4), leading to chromatin compaction and epigenetic repression of transcription.
• It represses MEF2-dependent transcription, playing critical roles in cell cycle progression, developmental events, and differentiation.
• Isoform 3 (MITR) lacks catalytic residues and is deacetylase-inactive; it represses transcription by recruiting HDAC1 and/or HDAC3, and inhibits skeletal myogenesis while protecting neurons from apoptosis.
• Encoded by the HDAC9 gene, also known as HDAC7B, HDRP, MITR; belongs to the class IIa histone deacetylase family.
• Localized to the nucleus.
• Post-translational modifications include phosphorylation and ubiquitination (Ubl conjugation), and it binds zinc as a metal cofactor.
• Broadly expressed, with highest levels in brain, heart, muscle, and testis; isoform 3 detected in bladder carcinoma cells.
• Keywords: chromatin regulator, transcription repressor, hydrolase, metal-binding, phosphoprotein, alternative splicing, chromosomal rearrangement.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1-100 of human HDAC9 (NP_478056.1); it may recognize full-length HDAC9 and isoforms sharing this N-terminal region. Verify specificity using appropriate controls and cell lines expressing distinct isoforms.
• For western blotting (WB), expect a band of approximately 111 kDa for the full-length protein. Smaller bands (~60 kDa) may correspond to isoforms such as MITR or cleavage products; validate with size markers and known expression patterns.
• In immunohistochemistry (IHC-P), nuclear staining is anticipated. Optimization of antigen retrieval and antibody dilution is recommended for each tissue type.
• For ELISA, the peptide immunogen can serve as a positive control, but cross-reactivity with related HDACs should be assessed due to sequence conservation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies this HDAC9 rabbit polyclonal antibody as part of our comprehensive one-stop IVD raw-material platform, supporting epigenetic research and diagnostic development from concept to clinic. We offer a wide range of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to streamline your assay workflows. Partner with us for reliable, high-quality materials that accelerate your projects in chromatin biology and beyond.