Anti-HDAC8 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9BY41

Core Product Specifications and Parameters

Biological Background: HDAC8 Function and Localization

• Histone deacetylase that catalyzes the deacetylation of lysine residues on core histones (H2A, H2B, H3, H4), contributing to epigenetic repression and transcriptional regulation. Related references: PMID:10748112 PMID:10922473 PMID:10926844
• Acts within large multiprotein complexes to influence cell cycle progression and developmental events. Related references: PMID:10748112 PMID:10922473 PMID:10926844
• Deacetylates the cohesin complex protein SMC3, regulating the release of cohesin from chromatin. Related references: PMID:22885700
• May play a role in smooth muscle cell contractility. Related references: PMID:15772115
• Also functions as a protein-lysine decrotonylase, removing crotonyl groups from histones. Related references: PMID:28497810
• Localizes to the nucleus and chromosomes, with additional presence in the cytoplasm.
• Exhibits weak expression in most tissues; higher levels found in heart, brain, kidney, pancreas, liver, lung, placenta, and prostate.
• Associated with disease variants linked to intellectual disability and obesity (UniProt Keywords: Disease variant, Intellectual disability, Obesity).

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant protein covering amino acids 1-377 of full-length human HDAC8; it is polyclonal and recognizes human, mouse, and rat HDAC8.
• For Western blot, the predicted molecular weight is 42 kDa. Validate specificity using relevant cell or tissue lysates.
• In immunohistochemistry and immunofluorescence, optimize fixation and permeabilization conditions for your sample type.
• Since this is a polyclonal antibody, verify lot-to-lot consistency for critical quantitative experiments.
• Knockout validation (as indicated by the product label) can be used to confirm antibody specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw materials, offering a comprehensive range of antibodies and reagents for epigenetic and chromatin research. This anti-HDAC8 polyclonal antibody, along with validated antibody pairs, specialized detection reagents, and bulk ancillary materials, supports your diagnostic development pipeline from concept to clinic. Contact us to streamline sourcing and accelerate your project timelines.