Anti-HDAC7 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q8WUI4

Core Product Specifications and Parameters

Biological Background: HDAC7 Function and Localization

• HDAC7 is a class IIa histone deacetylase encoded by the HDAC7 gene, functioning as a Zn²⁺-dependent hydrolase that removes acetyl groups from lysine residues on histone tails and non-histone proteins.
• It deacetylates core histones (H2A, H2B, H3, H4), leading to chromatin compaction and transcriptional repression via large multiprotein complexes; this activity is crucial for cell cycle progression and development (By similarity).
• In muscle differentiation, HDAC7 represses myocyte enhancer factor 2 (MEF2) transcription factors; upon differentiation signals, it shuttles from the nucleus to the cytoplasm, relieving repression and allowing muscle gene expression (By similarity).
• HDAC7 contributes to Epstein-Barr virus (EBV) latency by repressing the viral BZLF1 immediate-early gene. Related references: PMID:12239305
• It positively regulates the transcriptional repressor activity of FOXP3, a key factor in regulatory T cell function. Related references: PMID:17360565
• HDAC7 acts as a corepressor of retinoic acid receptor alpha (RARA), facilitating RARA deacetylation and inhibiting its DNA binding; this interaction contributes to microRNA-10a repression and modulation of inflammatory responses. Related references: PMID:28167758
• Beyond histones, HDAC7 deacetylates non-histone substrates such as the RNA demethylase ALKBH5, expanding its regulatory scope. Related references: PMID:37369679
• The protein shuttles between the nucleus and cytoplasm depending on phosphorylation status and cellular context; it undergoes alternative splicing and is subject to phosphorylation, underscoring its dynamic regulation (Keywords: Alternative splicing, Phosphoprotein, Nucleus, Cytoplasm).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 400–500 of human HDAC7; the antibody may recognize epitopes within this central region, which is conserved across human, mouse, and rat, consistent with the reported cross-reactivity.
• For western blot, the predicted molecular weight is approximately 103 kDa; consider using appropriate gel percentage and transfer conditions for high-molecular-weight proteins.
• In IHC-P and IF/ICC, fixation and permeabilization protocols should be optimized to preserve both nuclear and cytoplasmic localization; antigen retrieval may be necessary for formalin-fixed tissues.
• For ELISA, the peptide immunogen or recombinant HDAC7 protein can serve as coating antigen; the antibody may be suitable for both direct and indirect assay formats.
• Validate the antibody in the relevant sample system (e.g., cell lines with known HDAC7 expression, muscle or immune tissues) using appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined procurement channel for IVD raw materials, technical services, and consulting—supporting projects from concept to clinic. For epigenetic and transcriptional regulation research, we provide validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target sourcing. By integrating HDAC7 antibodies into your workflow, you gain access to our comprehensive supply capabilities, ensuring consistency and reliability for your assay development and production needs.