Anti-HDAC6 Rabbit Polyclonal Antibody - Q9UBN7

Core Product Specifications and Parameters

Biological Background: HDAC6 Function and Localization

• Core function: Deacetylates non-histone substrates, including tubulin, CTTN, SQSTM1, MSH2, MLH1, and RIGI. Plays key roles in cell motility, autophagy, protein quality control, and DNA repair.
• Central role in microtubule dynamics: Mediates tubulin deacetylation, destabilizing microtubules; essential for cilia disassembly. Related references: PMID:12024216 PMID:20308065 PMID:26246421 PMID:17604723
• Involved in autophagy: Deacetylates CTTN to promote autophagosome-lysosome fusion; deacetylates SQSTM1. Related references: PMID:30538141 PMID:31857589
• Aggresome pathway: Transports misfolded polyubiquitinated proteins to aggresomes for autophagic degradation. Related references: PMID:17846173
• DNA damage response: Deacetylates MSH2 and MLH1, reducing mismatch repair activity and promoting DNA damage tolerance. Related references: PMID:24882211 PMID:30770470
• Subcellular localization: Present in cytoplasm, cytoskeleton, nucleus, cilia, centrosome, dendrites, axons; shuttles between compartments depending on stimuli.
• Valine sensor: In valine deprivation, translocates to nucleus, deacetylates TET2, causing DNA demethylation. Related references: PMID:39567688
• Also acts as an E3 ubiquitin ligase, catalyzing ubiquitination of substrates like MSH2. Contains zinc-finger domain; regulated by phosphorylation and methylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 836-1104 (C-terminal region) of human HDAC6, suggesting that the antibody recognizes epitopes in this region. Consider potential isoform specificity.
• Predicted molecular weight is 131 kDa; verify band size in WB under reducing conditions.
• For IHC-P and IF/ICC, optimize antigen retrieval and permeabilization conditions. HDAC6 localizes to cytoplasm and microtubules, so staining patterns should be consistent.
• The antibody is reported to cross-react with mouse and rat; validate in the relevant sample system.
• For IP, the polyclonal nature may enhance capture efficiency but could also co-immunoprecipitate interacting partners; include appropriate controls.
• Consider testing different blocking conditions to reduce nonspecific binding in ELISA.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw material sourcing, offering validated antibodies, recombinant proteins, and ancillary reagents for diagnostic development. From epigenetics to autophagy, our portfolio supports your HDAC6-related research with bulk supply capabilities and rare-target sourcing. Contact us to accelerate your project from concept to clinic.