Anti-HDAC6 Monoclonal Antibody for WB, IHC-P, IP, ELISA - Q9UBN7

Core Product Specifications and Parameters

Biological Background: HDAC6 Function and Localization

• HDAC6 functions primarily as a non-histone protein deacetylase and also possesses E3 ubiquitin ligase activity, targeting diverse substrates to regulate cellular processes (PubMed:12024216, PubMed:18606987, PubMed:20308065). Related references: PMID:12024216 PMID:18606987 PMID:20308065
• It plays a critical role in microtubule dynamics by deacetylating alpha-tubulin, promoting microtubule destabilization and cilia disassembly, which is essential for cell motility (PubMed:12024216, PubMed:20308065, PubMed:26246421, PubMed:17604723). Related references: PMID:12024216 PMID:20308065 PMID:26246421 PMID:17604723
• HDAC6 facilitates autophagy and aggresome formation; it deacetylates cortactin (CTTN) to promote autophagosome-lysosome fusion and deacetylates SQSTM1/p62, while also targeting polyubiquitinated misfolded proteins to aggresomes for degradation (PubMed:30538141, PubMed:31857589, PubMed:17846173). Related references: PMID:30538141 PMID:31857589 PMID:17846173
• It deacetylates DNA mismatch repair proteins MSH2 and MLH1, leading to their degradation or impaired complex formation, thereby reducing DNA repair capacity and promoting tolerance to DNA damage (PubMed:24882211, PubMed:30770470). Related references: PMID:24882211 PMID:30770470
• HDAC6 localizes to the cytoplasm, nucleus, centrosome, cilium, and neuronal projections, reflecting its multifaceted functions in different cellular compartments.
• The protein contains a primate-specific SE14 repeat region that acts as a valine sensor; under valine deprivation, HDAC6 translocates to the nucleus to deacetylate TET2, promoting DNA demethylation (PubMed:39567688). Related references: PMID:39567688
• During SARS-CoV-2 infection, HDAC6 deacetylates the viral N protein, facilitating disruption of stress granules and enhancing viral replication.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (aa 1–100) of human HDAC6; the antibody may recognize epitopes within this domain.
• For Western blotting, the predicted molecular weight is 131 kDa; ensure adequate separation of high-molecular-weight proteins and include appropriate positive and negative controls.
• When using immunohistochemistry (IHC-P), validate the antibody on known positive human tissue samples and consider antigen retrieval conditions for optimal staining.
• In immunoprecipitation and ELISA, titrate the antibody concentration and confirm specificity with recombinant HDAC6 or cell lysates overexpressing the target.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material platform, offering validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents to streamline assay development. This anti-HDAC6 monoclonal antibody is part of our portfolio targeting key epigenetic and autophagy regulators, supporting research and diagnostic applications in oncology and neurodegeneration. Leverage CamelBio’s one-stop sourcing to secure rare-target reagents and accelerate your project from concept to clinic.