Anti-HDAC6 Monoclonal Antibody for WB, ELISA - Q9UBN7

Core Product Specifications and Parameters

Biological Background: HDAC6 Function and Localization

• HDAC6 (histone deacetylase 6) is a cytoplasmic class II deacetylase, encoded by the HDAC6 gene (Gene ID: 10013), and also functions as an E3 ubiquitin-protein ligase. It is known by aliases HD6, JM21, CPBHM, and PPP1R90, and features two catalytic domains and a C-terminal ubiquitin-binding zinc-finger domain.
• HDAC6 primarily deacetylates a wide range of non-histone substrates, including α-tubulin, cortactin (CTTN), peroxiredoxins (PRDX1/2), SQSTM1, and transcription factors, thereby regulating microtubule dynamics, autophagy, oxidative stress response, and gene expression. Related references: PMID:12024216 PMID:18606987 PMID:20308065 PMID:30538141 PMID:31857589 PMID:38534334
• It plays a critical role in microtubule-dependent cell motility by deacetylating α-tubulin at lysine 40, which destabilizes dynamic microtubules and promotes cilia disassembly. Related references: PMID:17604723 PMID:26246421
• In the aggresome-autophagy pathway, HDAC6 recognizes polyubiquitinated misfolded proteins and transports them to the aggresome, facilitating their clearance by autophagy and contributing to proteostasis. Related references: PMID:17846173
• HDAC6 modulates DNA damage tolerance by deacetylating and ubiquitinating DNA mismatch repair proteins MSH2 and MLH1, leading to their degradation and reduced repair activity, with implications for chemoresistance. Related references: PMID:24882211 PMID:30770470
• As a valine sensor, HDAC6 binds valine via its primate-specific SE14 repeats; under valine deprivation, it translocates to the nucleus to deacetylate TET2, inducing DNA demethylation and damage. Related references: PMID:39567688
• HDAC6 localizes to the cytoplasm, cytoskeleton (microtubules, centrosome, basal body), nucleus, and cell projections (cilia, dendrites, axons), consistent with its roles in cell motility, signaling, and protein trafficking.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide from amino acids 600–700 of human HDAC6, located within the second catalytic domain. This region is well-conserved and likely contributes to epitope specificity. Consider using this antibody for detecting both full-length HDAC6 and potential isoforms or degradation fragments.
• Recommended applications include Western blotting (WB) and ELISA based on supplier data. In WB, a band at ~131 kDa is expected under standard reducing conditions. Validate in relevant human cell lines (e.g., HeLa, HEK293) and optimize lysate preparation to preserve HDAC6 stability.
• Cross-reactivity has been confirmed for human HDAC6. For use with other species, check sequence homology of the immunogen region and perform a pilot experiment.
• Optimize antibody dilution, incubation time, and detection system according to your specific experimental setup. Including appropriate positive and negative controls is essential for confirming specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, providing a streamlined pathway from research concept to clinical application. This anti-HDAC6 rabbit monoclonal antibody is an example of our offerings in the epigenetics and cancer research area. Beyond individual antibodies, we supply validated antibody pairs, optimized bulk reagents, and can assist with the acquisition of rare-target raw materials, supporting your assay development needs.