Anti-HDAC6 (Hd6, Sfc6, Hdac5, mHDA2) Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - Q9Z2V5

Core Product Specifications and Parameters

Biological Background: HDAC6 Function and Localization

• HDAC6 (histone deacetylase 6) is a cytoplasmic enzyme that deacetylates a wide range of non-histone substrates, including α-tubulin, cortactin, and peroxiredoxins, thereby regulating microtubule dynamics, cell motility, and redox homeostasis. Related references: PMID:12606581, PMID:19893491
• It plays a central role in microtubule-dependent cell motility by deacetylating α-tubulin, which destabilizes dynamic microtubules and is required for cilia disassembly. Related references: PMID:12486003, PMID:27737934
• HDAC6 acts as an adapter that recognizes polyubiquitinated misfolded proteins and targets them to the aggresome, facilitating their clearance by autophagy. Related references: PMID:22819792
• The enzyme deacetylates the antiviral protein RIG-I, a modification required for viral RNA detection, and also regulates PRDM16 to control brown adipocyte function. Related references: PMID:26746851, PMID:35075301
• HDAC6 localizes predominantly to the cytoplasm but is also found in the nucleus, centrosome, basal body, and neuronal projections including dendrites, axons, and cilia.
• In mice, HDAC6 is expressed in cortical neurons, Purkinje cells, and keratinocytes (at protein level).
• The protein contains two catalytic deacetylase domains and a C-terminal ubiquitin-binding zinc-finger domain, classifying it as a class IIb HDAC with roles in transcription regulation and chromatin remodeling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 836–1037) of mouse HDAC6, which includes part of the second deacetylase domain and the ubiquitin-binding zinc finger. This design may favor detection of full-length HDAC6 and potentially specific isoforms.
• This rabbit monoclonal antibody offers batch-to-batch consistency and has been qualified for WB, IF-P, IHC-P, and ELISA by the supplier.
• Given the large predicted protein size (~126 kDa), consider using low-percentage SDS-PAGE gels (e.g., 6–8%) and extended transfer times for western blotting. A high-molecular-weight marker is recommended.
• For immunofluorescence or immunohistochemistry, optimize permeabilization and fixation conditions, as HDAC6 is both cytoplasmic and nuclear. Microtubule-associated staining may benefit from anti-tubulin co-staining.
• Cross-reactivity has been confirmed in mouse and rat; additional species should be validated independently.

CamelBio: Your One-Stop Sourcing Bridge

As a comprehensive IVD raw-materials provider, CamelBio offers researchers and diagnostic manufacturers streamlined access to validated antibody pairs, optimized monoclonal antibodies like this HDAC6 clone, bulk ancillary reagents, and specialized sourcing for rare targets. Our expertise in HDAC6 biology and protein degradation pathways enables us to support your assay development from concept to clinical application. Contact our team to discuss your specific sourcing needs.