Anti-HDAC5 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9UQL6

Core Product Specifications and Parameters

Biological Background: HDAC5 Function and Localization

• HDAC5 catalyzes the deacetylation of N-terminal lysine residues on core histones H2A, H2B, H3, and H4 in vitro, a key step in establishing epigenetic repression. Related references: PMID:10220385
• Through histone deacetylation, HDAC5 contributes to transcriptional regulation, cell cycle progression, and developmental processes, typically as part of large multiprotein complexes.
• HDAC5 represses transcription of the myocyte enhancer factor MEF2C; during muscle differentiation, it shuttles to the cytoplasm, thereby derepressing myogenic genes.
• In breast cancer, HDAC5 is involved in MTA1-mediated epigenetic silencing of ESR1 (estrogen receptor alpha) expression.
• HDAC5 functions as a corepressor of the retinoic acid receptor alpha (RARA), mediating its deacetylation and contributing to the repression of microRNA-10a in inflammatory responses. Related references: PMID:28167758
• The protein localizes to both the nucleus and the cytoplasm.
• HDAC5 is widely expressed, with detected expression in heart, brain, kidney, pancreas, liver, skeletal muscle, and placenta. Related references: PMID:10220385
• Post-translational modifications including phosphorylation and acetylation, along with alternative splicing, regulate HDAC5 function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an internal region (amino acids 500–600) of human HDAC5; consider sequence alignment to verify specificity for your target species and isoform.
• For Western blot, the predicted molecular weight is 122 kDa; note that post-translational modifications may cause band shifts.
• This antibody reacts with human, mouse, and rat; for other species, validate cross-reactivity experimentally.
• Suitable for multiple applications (WB, IHC-P, IF/ICC, ELISA); optimize dilutions and conditions for your specific assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop partner for IVD raw materials, technical services, and consulting from concept to clinic. This HDAC5 antibody exemplifies our portfolio of optimized polyclonal antibodies for epigenetic and transcriptional regulation research. We also offer validated antibody pairs, bulk ancillary reagents, and sourcing assistance for rare targets to support your full development pipeline.