Anti-HDAC4 Polyclonal Antibody for WB, IHC-P, ELISA - P56524

Core Product Specifications and Parameters

Biological Background: HDAC4 Function and Localization

• HDAC4 is a class IIa histone deacetylase that represses transcription by removing acetyl groups from N-terminal lysines of core histones (H2A, H2B, H3, H4), promoting chromatin condensation.
• Through its N-terminal extension, HDAC4 interacts with MEF2 transcription factors (MEF2A, MEF2C, MEF2D), regulating muscle differentiation and hypertrophy.
• In breast cancer, HDAC4 participates in MTA1-mediated epigenetic silencing of ESR1, contributing to hormonal therapy resistance.
• HDAC4 deacetylates HSPA1A and HSPA1B at lysine 77, enhancing their interaction with co-chaperone STUB1 and influencing protein quality control. Related references: PMID:27708256
• HDAC4 shuttles between the nucleus and cytoplasm; phosphorylation by kinases such as CaMK promotes 14-3-3 binding and cytoplasmic retention, while sumoylation and ubiquitination regulate its stability and localization.
• The enzyme is zinc-dependent, with a conserved catalytic domain that coordinates Zn²⁺; mutations disrupting metal binding impair activity.
• Alternative splicing of HDAC4 generates isoforms with distinct tissue-specific expression and regulatory functions.

Experimental Guidance and Technical Tips

• The immunogen spans residues 451–650, located within the conserved deacetylase domain; this region is >95% identical across human, mouse, and rat, explaining the broad cross-reactivity.
• For Western blot (WB), a band at ~119 kDa is expected; use nuclear-enriched lysates (e.g., HeLa, C2C12) and validate with appropriate positive and negative controls.
• In immunohistochemistry (IHC-P), antigen retrieval conditions (e.g., citrate buffer pH 6.0) and primary antibody dilution must be optimized; both nuclear and cytoplasmic staining patterns are possible depending on tissue and disease state.
• For ELISA, the antibody may be used as a capture or detection reagent; pairing with a validated HDAC4 detection antibody is recommended for sandwich assays.
• The polyclonal nature offers robust signal amplification, but lot-to-lot consistency should be verified for quantitative applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a premier one-stop supplier for IVD raw materials, providing research and diagnostic communities with high-quality antibodies, antigens, and ancillary reagents. Our HDAC4 Rabbit pAb supports epigenetic biomarker discovery, cancer diagnostic development, and protein deacetylase activity studies. We offer customized sourcing for rare targets, bulk production, and technical consulting to accelerate your assay development from concept to clinic.