Anti-HDAC4 Polyclonal Antibody for WB, ELISA - P56524

Core Product Specifications and Parameters

Biological Background: HDAC4 Function and Localization

• HDAC4 is a histone deacetylase that catalyzes the removal of acetyl groups from N-terminal lysines of core histones (H2A, H2B, H3, H4), contributing to chromatin compaction and epigenetic repression of transcription.
• It functions within large multiprotein complexes and is implicated in the regulation of cell cycle progression and developmental processes.
• In muscle development, HDAC4 interacts with myocyte enhancer factor‑2 (MEF2) proteins (MEF2A, MEF2C, MEF2D) to repress muscle‑specific gene expression, a key mechanism in muscle maturation.
• HDAC4 participates in the MTA1‑mediated epigenetic silencing of the estrogen receptor gene (ESR1) in breast cancer, linking it to tumor progression.
• Beyond histone substrates, HDAC4 deacetylates the heat shock proteins HSPA1A and HSPA1B at Lys‑77, which promotes their association with the co‑chaperone STUB1. Related references: PMID:27708256
• The protein localizes to both the nucleus and the cytoplasm, enabling it to regulate transcription and cytoplasmic signaling pathways.
• HDAC4 features structural motifs including a coiled‑coil domain and zinc‑binding sites, and its activity is modulated by post‑translational modifications such as phosphorylation, ubiquitination, and isopeptide bond formation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 530–630 of human HDAC4. This sequence can be used for peptide competition assays or as a reference for epitope mapping.
• For western blotting, consider using total cell lysates or fractionated nuclear and cytoplasmic extracts, as HDAC4 is present in both compartments.
• ELISA conditions may be optimized using recombinant HDAC4 or synthetic peptide standards, and the background matrix should match the intended sample type.
• Cross‑reactivity with other HDAC family members should be assessed experimentally, especially when working with tissues or cells expressing multiple HDACs.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw‑material procurement for diagnostic developers and research laboratories. For epigenetics and transcription‑focused projects involving HDAC4, we offer validated antibody pairs, bulk ancillary reagents, and customized monoclonal/polyclonal antibody development. Beyond this product, CamelBio sources rare targets and supports end‑to‑end assay development, helping you accelerate from concept to clinic.