Anti-HDAC3 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O15379

Core Product Specifications and Parameters

Biological Background: HDAC3 Function and Localization

• HDAC3 is a class I histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal tails of core histones (H2A, H2B, H3, H4), leading to a condensed chromatin structure and epigenetic transcriptional repression.
  Related references: PMID:21030595, PMID:23911289
• It functions as part of multiprotein repressor complexes such as N-Cor, and is recruited by transcription factors like BCL6 to deacetylate H3K27 at enhancer elements, antagonizing EP300 activity.
  Related references: PMID:23911289, PMID:22230954
• Beyond histones, HDAC3 deacetylates a range of non-histone substrates including RARA, STAT3, MAPK14, and MEF2D, affecting pathways from inflammation to cell survival.
  Related references: PMID:15653507, PMID:21030595, PMID:21444723, PMID:28167758
• HDAC3 also acts as a protein-lysine deacylase, removing non-acetyl acyl modifications such as crotonyl (decrotonylation), lactyl (delactylation), and 2-hydroxyisobutyryl groups, with substrates like MAPRE1/EB1 and NBN/NBS1.
  Related references: PMID:28497810, PMID:29192674, PMID:34608293, PMID:38961290
• In the circadian clock, HDAC3 has deacetylase-independent roles: it promotes BMAL1 ubiquitination and stabilizes CRY proteins, thereby regulating circadian gene expression and hepatic lipid metabolism.
• Subcellularly, HDAC3 is predominantly nuclear and chromosome-associated, but also detected in the cytoplasm, consistent with its shuttling roles.
• Widely expressed across tissues, HDAC3 is a phosphoprotein with alternative splicing and multiple interaction partners, underlying its involvement in diverse biological processes.

Experimental Guidance and Technical Tips

• The immunogen (aa 299–428) targets the C-terminal region of human HDAC3, which is conserved in mouse and rat (as indicated by cross-reactivity data); verify cross-reactivity in your specific model system.
• In Western blot, HDAC3 has a predicted molecular weight of ~49 kDa; use appropriate protein standards and confirm band specificity with siRNA knockdown or KO cell lysates if available.
• For IHC-P, antigen retrieval methods (e.g., citrate buffer pH 6.0, heat-induced) may be required; test a range of antibody dilutions (e.g., 1:50–1:200) on control tissues.
• For IF/ICC, fix cells with 4% PFA and permeabilize with 0.1% Triton X-100; nuclear localization should be observed; include secondary antibody-only controls.
• For ELISA, the antibody can be used in direct or indirect formats; titrate against recombinant HDAC3 protein to determine optimal coating and detection concentrations.
• Store antibody at -20°C in aliquots; avoid repeated freeze-thaw cycles to maintain performance.

CamelBio: Your One-Stop Sourcing Bridge

As a premier IVD raw material supplier, CamelBio provides diagnostic manufacturers and laboratories with convenient access to high-quality antibodies like this HDAC3 rabbit polyclonal antibody, ideal for chromatin and epigenetic research. Beyond single reagents, we offer validated antibody pairs, bulk monoclonal/polyclonal antibodies, ancillary proteins, and custom sourcing for rare targets, supporting your entire assay development workflow from discovery to validation.