Anti-HDAC3 Rabbit Monoclonal Antibody for WB, IP, ELISA - O15379

Core Product Specifications and Parameters

Biological Background: HDAC3 Function and Localization

• HDAC3 is a histone deacetylase that removes acetyl groups from lysine residues on core histones (H2A, H2B, H3, H4) and non-histone substrates, playing a central role in epigenetic repression, transcriptional regulation, cell cycle progression, and development. Related references: PMID:21030595, PMID:21444723, PMID:23911289

• It operates within large multiprotein complexes such as the N-CoR repressor complex, which activates its deacetylase activity, and participates in BCL6-mediated transcriptional repression by deacetylating H3K27 on enhancer elements. Related references: PMID:23911289, PMID:22230954

• Regulates both the activation and repression phases of the circadian clock in a deacetylase activity-independent manner, involving BMAL1 ubiquitination and CRY1/BMAL1 interaction (By similarity).

• Contributes to endothelial cell survival under oxidative stress by activating HMOX1 transcription via XBP1 isoform 1 and a PI(3)K/mTORC2/Akt-dependent signaling pathway. Related references: PMID:25190803

• Deacetylates a range of non-histone proteins, including KAT5, MEF2D, MAPK14, RARA, and STAT3, thereby extending its regulatory functions beyond chromatin. Related references: PMID:15653507, PMID:21030595, PMID:21444723

• Acts as a corepressor of RARA, mediating its deacetylation and inhibiting RARE DNA element binding; involved in the inflammatory response by repressing microRNA-10a expression. Related references: PMID:28167758

• Additionally functions as a protein-lysine deacylase, capable of removing crotonyl, lactoyl, 2-hydroxyisobutyryl, and isonicotinyl groups; catalyzes decrotonylation of MAPRE1/EB1 and delactylation of NBN/NBS1, the latter inhibiting DNA double-strand breaks via homologous recombination. Related references: PMID:28497810, PMID:29192674, PMID:34608293, PMID:38961290

• Localizes primarily in the nucleus but also found in the chromosome, cytoplasm, and cytosol; it is widely expressed across tissues. Post-translational modifications include phosphorylation and ubiquitin conjugation, and alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 329–428 of human HDAC3; consider confirming antibody specificity in your experimental model using relevant controls.
• Validated applications include Western blot (WB), immunoprecipitation (IP), and ELISA. The predicted molecular weight is 49 kDa; optimize gel electrophoresis and transfer conditions for WB.
• Cross-reactivity is observed with human, mouse, and rat samples, suggesting conserved epitope recognition; verify performance in other species if required.
• For IP, titrate the antibody concentration and include appropriate negative and positive controls to ensure pull-down specificity.
• Given HDAC3’s association with various corepressor complexes, this antibody may be suitable for co-immunoprecipitation studies to analyze protein-protein interactions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This HDAC3 monoclonal antibody is part of our extensive portfolio of validated antibodies for epigenetic and transcriptional research. We also offer bulk ordering, customized antibody development, and rare target sourcing to support your IVD and research projects.