Anti-HDAC3 Rabbit Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - O15379

Core Product Specifications and Parameters

Biological Background: HDAC3 Function and Localization

• HDAC3 is a histone deacetylase that removes acetyl groups from lysine residues on core histones (H2A, H2B, H3, H4) and non-histone proteins, contributing to epigenetic repression and transcriptional regulation.
  Related references: PMID:21030595 PMID:21444723 PMID:23911289
• It functions within large multiprotein complexes such as the N-Cor repressor complex to modulate gene expression and plays a role in BCL6-mediated transcriptional repression by deacetylating H3K27 on enhancer elements.
  Related references: PMID:23911289 PMID:22230954
• Beyond histones, HDAC3 deacetylates non-histone targets including KAT5, MEF2D, MAPK14, RARA, and STAT3, influencing diverse cellular processes such as inflammation and circadian rhythm.
  Related references: PMID:15653507 PMID:21030595 PMID:21444723
• It also acts as a protein-lysine deacylase, removing acyl groups like crotonyl, lactyl, 2-hydroxyisobutyryl, and isonicotinyl from lysine residues, thus mediating decrotonylation of MAPRE1/EB1 and delactylation of NBN/NBS1 to regulate DNA repair via homologous recombination.
  Related references: PMID:28497810 PMID:29192674 PMID:34608293 PMID:38961290
• In endothelial cells, HDAC3 contributes to NFE2L2-mediated HMOX1 expression via a PI(3)K/mTORC2/Akt-dependent pathway, promoting survival under oxidative stress.
  Related references: PMID:25190803
• Subcellularly, HDAC3 localizes primarily to the nucleus and chromosomes but is also found in the cytoplasm and cytosol, consistent with its roles in chromatin regulation and signal transduction.
• It is widely expressed across tissues and is subject to post-translational modifications including phosphorylation and ubiquitination, as indicated by keywords: Phosphoprotein, Ubl conjugation.
• HDAC3 plays a critical role in the circadian clock by regulating BMAL1 ubiquitination and CRY interactions in a deacetylase-independent manner, and the NCOR1-HDAC3 complex controls circadian expression of clock genes and lipid metabolism genes in the liver.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (amino acids 299-428) of human HDAC3, which is highly conserved; consider sequence alignment when assessing cross-reactivity with mouse, rat, and monkey.
• For Western blotting, an expected band at ~49 kDa is predicted; validate in positive control lysates from widely expressing cell lines (e.g., HeLa, Jurkat).
• In immunofluorescence and immunohistochemistry, HDAC3 typically shows nuclear and cytoplasmic localization; fixation and permeabilization conditions may need optimization for specific cell types or tissues.
• The antibody is compatible with ELISA; titration experiments are recommended to establish optimal working concentrations for each assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop sourcing partner for IVD raw materials, from concept to clinic. For HDAC3 and other epigenetic targets, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk reagents, and rare target sourcing to streamline diagnostic development.