Anti-HDAC3 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O15379

Core Product Specifications and Parameters

Biological Background: HDAC3 Function and Localization

• Acts as a histone deacetylase, catalyzing the deacetylation of lysine residues on core histones (H2A, H2B, H3, H4) and various non-histone proteins, leading to epigenetic repression and playing critical roles in transcriptional regulation, cell cycle progression, and developmental events. Related references: PMID:21030595, PMID:21444723, PMID:23911289
• Functions within large multiprotein complexes such as the N-Cor repressor complex and participates in BCL6-mediated transcriptional repression by deacetylating H3K27 on enhancer elements, antagonizing EP300 acetyltransferase activity. Related references: PMID:23911289, PMID:22230954
• Deacetylates non-histone substrates, including KAT5, MEF2D, MAPK14, RARA, and STAT3, thereby modulating their activities in diverse cellular signaling pathways. Related references: PMID:15653507, PMID:21030595, PMID:21444723
• Also acts as a protein-lysine deacylase, catalyzing the removal of crotonyl, lactoyl, 2-hydroxyisobutyryl, and isonicotinyl modifications; for instance, decrotonylates MAPRE1 and delactylates NBN, affecting DNA double-strand break repair. Related references: PMID:28497810, PMID:29192674, PMID:34608293, PMID:38961290
• Contributes to circadian clock regulation in a deacetylase-independent manner, promoting BMAL1 ubiquitination during activation and blocking CRY1/2 degradation during repression; also influences hepatic lipid metabolism gene expression.
• Localizes primarily in the nucleus and on chromosomes, with additional presence in the cytoplasm and cytosol, consistent with its transcriptional regulatory and shuttling functions.
• Widely expressed across multiple tissues, reflecting its fundamental role in epigenetic control.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide covering amino acids 329–428 of human HDAC3 (C-terminal region). When extending the use to other species, confirm sequence homology in this region.
• Optimize antibody concentration for each assay. For Western blotting, consider a starting dilution of 1:500–1:2000, and include appropriate positive and negative controls. For immunohistochemistry, antigen retrieval may improve detection.
• This antibody has shown reactivity with human, mouse, and rat HDAC3. If used with other species, perform cross-reactivity validation.
• The predicted molecular weight is 49 kDa; verify the band size in your experimental system, as post-translational modifications may cause subtle shifts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports your HDAC3-focused diagnostic and research initiatives with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our integrated sourcing model streamlines procurement from discovery through clinical application, ensuring consistent supply of high-quality reagents for chromatin biology and epigenetic target studies.