Anti-HDAC2 Rabbit Monoclonal Antibody for WB, ELISA - Q92769

Core Product Specifications and Parameters

Biological Background: HDAC2 Function and Localization

• HDAC2 (Histone deacetylase 2) is a chromatin-modifying enzyme that catalyzes the removal of acetyl groups from lysine residues on core histones (H2A, H2B, H3, H4), leading to epigenetic transcriptional repression. Related references: PMID:28497810
• It forms multiprotein repressor complexes with transcriptional regulators including MAD, SIN3, YY1, and N-COR, mediating targeted gene silencing. Related references: PMID:12724404
• HDAC2 is a core component of the NuRD (Nucleosome Remodeling and Deacetylase) complex, which couples histone deacetylation with ATP-dependent chromatin remodeling to regulate gene expression. Related references: PMID:16428440, PMID:28977666
• An integral subunit of the SIN3B complex that specifically recognizes H3K27ac marks via PHF12 and counteracts EP300 acetyltransferase activity, highlighting its role in epigenetic crosstalk. Related references: PMID:37137925
• Beyond histones, HDAC2 deacetylates non-histone substrates such as TSHZ3 (a transcriptional repressor) and mediates MTA1-dependent corepression of TFF1 and CDKN1A, broadening its regulatory impact. Related references: PMID:19343227, PMID:21965678
• Exhibits protein deacylase activity, removing not only acetyl but also crotonyl, lactyl, and 2-hydroxyisobutyryl modifications, reflecting its broad substrate specificity. Related references: PMID:28497810, PMID:29192674, PMID:35044827
• HDAC2 localizes predominantly in the nucleus but can also be cytoplasmic, and is widely expressed across human tissues with lower abundance in brain and lung.

Experimental Guidance and Technical Tips

• For Western blotting, expect a specific band at approximately 55 kDa. Use nuclear-enriched lysates or whole-cell extracts with appropriate protease inhibitors for optimal detection.
• In ELISA, the antibody can be employed as a capture or detection reagent; perform checkerboard titrations to establish optimal working concentrations.
• Given cross-reactivity with mouse and rat orthologs, validate the antibody in the species and tissue of interest before large-scale experiments.
• Since the immunogen targets the N-terminus (aa 1-100), reducing SDS-PAGE conditions are recommended to ensure epitope accessibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for high-quality IVD raw materials, streamlining sourcing from target selection to commercial kit assembly. For epigenetic targets like HDAC2, we supply validated monoclonal antibodies, optimized antibody pairs, and bulk ancillary reagents to support your diagnostic development pipeline. Whether you need rare-target sourcing or scalable production of critical raw materials, CamelBio's integrated supply chain ensures seamless procurement and technical continuity.