Anti-HDAC11 Rabbit Polyclonal Antibody for WB, ELISA - Q96DB2

Core Product Specifications and Parameters

Biological Background: HDAC11 Function and Localization

• HDAC11 is a histone deacetylase responsible for removing acetyl groups from lysine residues on the N-terminal tails of core histones H2A, H2B, H3, and H4.
• By deacetylating histones, HDAC11 contributes to chromatin compaction and epigenetic gene silencing, playing a role in transcriptional repression.
• HDAC11 is involved in the regulation of cell cycle progression and developmental processes through its histone-modifying activity.
• HDAC11 functions within large multiprotein complexes to mediate its deacetylase activity on chromatin.
• The protein localizes predominantly to the nucleus, consistent with its role in histone modification and transcriptional regulation.
• HDAC11 is weakly expressed in most tissues, with notably higher expression in brain, heart, skeletal muscle, kidney, and testis.
• Additional keywords indicate HDAC11 undergoes alternative splicing and is classified as a chromatin regulator, hydrolase, and transcriptional repressor.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 270-347 of human HDAC11, covering the C-terminal region, which may provide specificity for detecting full-length HDAC11 in western blotting.
• For WB, consider using nuclear extracts or whole-cell lysates from human, mouse, or rat cell lines, as cross-reactivity with these species is indicated.
• Validate antibody specificity by comparing signals in knockdown or knockout samples, or by using appropriate controls, as this is a polyclonal antibody.
• For ELISA, the recombinant protein-based immunogen design suggests suitability for detecting denatured or native protein, but optimal coating conditions should be determined empirically.
• Due to the 39 kDa predicted molecular weight, ensure sufficient protein transfer and use molecular weight markers for accurate band identification in WB.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to premium IVD raw materials, including validated antibodies for epigenetic targets such as HDAC11. Our one-stop sourcing platform offers polyclonal antibodies, antigen-matched antibody pairs, and bulk ancillary reagents to accelerate your assay development and research into histone modifications and transcriptional regulation.