Anti-HDAC11 Rabbit Monoclonal Antibody for WB, ELISA - Q96DB2

Core Product Specifications and Parameters

Biological Background: HDAC11 Function and Localization

• HDAC11 (Histone deacetylase 11) is a member of the class IV histone deacetylase family, functioning as a chromatin regulator and transcriptional repressor.
• It catalyzes the deacetylation of lysine residues on the N-terminal tails of core histones (H2A, H2B, H3, and H4), leading to epigenetic silencing.
• This deacetylation activity is essential for modulating chromatin structure and regulating gene expression, impacting processes such as cell cycle progression and development.
• HDAC11 operates within large multiprotein complexes to exert its repressive effects on transcription.
• The protein is predominantly localized in the nucleus, consistent with its role in histone modification.
• Expression is tissue-specific; it is strongly expressed in the brain, heart, skeletal muscle, kidney, and testis, with weaker expression in other tissues.
• Alternative splicing of the HDAC11 gene results in multiple transcript variants, contributing to functional diversity.
• Key post-translational modifications and molecular functions are reflected in its association with keywords: hydrolase, chromatin regulator, and repressor.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length recombinant HDAC11 (amino acids 1-347), which may recognize all known isoforms. Validate antibody specificity using appropriate controls in your target tissue or cell line.
• For Western blot (WB), the predicted molecular weight is approximately 39 kDa. Optimize electrophoresis and transfer conditions for clear detection of this relatively small protein.
• In ELISA applications, consider using a matched antibody pair design and validate linearity and sensitivity with recombinant HDAC11 protein.
• Since HDAC11 is a nuclear protein, ensure efficient nuclear extraction in your sample preparation to maximize signal.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This HDAC11 antibody supports epigenetic research applications, and CamelBio offers a comprehensive portfolio of validated antibody pairs, bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development.