Anti-HDAC1 Rabbit Monoclonal Antibody for IHC-P, IF/ICC, ELISA - Q13547

Core Product Specifications and Parameters

Biological Background: HDAC1 Function and Localization

• HDAC1 catalyzes the deacetylation of lysine residues on the N-terminal tails of core histones H2A, H2B, H3, and H4, a modification critical for epigenetic repression and chromatin compaction.
  Related references: PMID:16762839, PMID:17704056, PMID:28497810

• It functions within large multiprotein complexes such as the NuRD and SIN3B complexes, which are recruited to chromatin to remodel structure and regulate transcription, cell cycle progression, and development.
  Related references: PMID:16428440, PMID:28977666, PMID:21041482, PMID:16762839, PMID:17704056

• In addition to histones, HDAC1 deacetylates non-histone targets such as RELA (NF-κB p65), SP1, SP3, STAT3, NR1D2, and TSHZ3, thereby modulating inflammatory signaling, transcription factor activity, and circadian rhythms.
  Related references: PMID:12837748, PMID:16285960, PMID:16337145, PMID:17000776, PMID:17996965, PMID:16478997

• HDAC1 exhibits protein decrotonylase and delactylase activities, removing crotonyl and lactyl groups from histones, expanding its role in the histone code.
  Related references: PMID:28497810, PMID:35044827

• It is a component of the BRG1-RB1-HDAC1 complex that negatively regulates CREST-mediated transcription in resting neurons; upon calcium stimulation, HDAC1 is released, allowing transcriptional activation.
  Related references: PMID:19081374

• Localized predominantly in the nucleus, HDAC1 is ubiquitously expressed with higher levels in heart, pancreas, and testis, and lower in kidney and brain.

Experimental Guidance and Technical Tips

• For IHC-P, antigen retrieval (e.g., heat-mediated in citrate buffer) is strongly recommended; validate the antibody in tissues known to express HDAC1, such as heart, pancreas, or testis.
• In IF/ICC, appropriate permeabilization and blocking steps should be optimized; expected localization is nuclear.
• For ELISA, the antibody can be used as a capture or detection reagent; cross-reactivity with mouse and rat HDAC1 allows for broader assay design.
• The immunogen lies within the C-terminal region (aa 350-450), which is conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• This monoclonal antibody, generated in rabbit, typically offers high specificity and low background; however, always include appropriate controls in your experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies a comprehensive range of IVD raw materials, including validated monoclonal antibodies like this HDAC1 antibody, along with optimized antibody pairs, bulk ancillary reagents, and rare-target sourcing for epigenetic and chromatin research applications. Whether you need consistent lot-to-lot performance for diagnostic kit development or specialized reagents for basic research, our one-stop procurement platform streamlines access from concept to clinic.