Anti-HDAC1 Polyclonal Antibody for WB, IP, IF/ICC, IHC-P, ELISA - Q13547

Core Product Specifications and Parameters

Biological Background: HDAC1 Function and Localization

• HDAC1 catalyzes the deacetylation of lysine residues on core histones (H2A, H2B, H3, H4), a pivotal mechanism in epigenetic transcriptional repression (PubMed:16762839, PubMed:17704056, PubMed:28497810). Related references: PMID:16762839, PMID:17704056, PMID:28497810
• It functions within large multiprotein complexes, including NuRD and SIN3B, to remodel chromatin and regulate gene expression (PubMed:16428440, PubMed:28977666, PubMed:21041482). Related references: PMID:16428440, PMID:28977666, PMID:21041482
• Beyond histones, HDAC1 deacetylates non-histone proteins such as transcription factors RELA, SP1, SP3, STAT3, and TSHZ3, thereby modulating their transcriptional activities (PubMed:12837748, PubMed:16285960, PubMed:16337145, PubMed:16478997, PubMed:17000776, PubMed:19343227). Related references: PMID:12837748, PMID:16285960, PMID:16337145, PMID:16478997, PMID:17000776, PMID:19343227
• In addition to deacetylation, HDAC1 possesses protein-lysine deacylase activity, enabling decrotonylation and delactylation of histones, expanding its epigenetic regulatory repertoire (PubMed:28497810, PubMed:35044827). Related references: PMID:28497810, PMID:35044827
• HDAC1 localizes exclusively to the nucleus, consistent with its direct role in chromatin modification and transcriptional control.
• It is ubiquitously expressed, with higher levels observed in heart, pancreas, and testis, and relatively lower levels in kidney and brain.
• Post-translational modifications, including acetylation, phosphorylation, methylation, S-nitrosylation, and ubiquitination, dynamically regulate HDAC1 function and interactions.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 393–482) lies within the C-terminal region of human HDAC1, which shares high sequence similarity with mouse and rat orthologs, consistent with the observed cross-reactivity.
• For Western blotting, using nuclear-enriched lysates may enhance detection; always titrate the antibody and include appropriate controls.
• In immunoprecipitation, confirm antibody compatibility with the chosen lysis buffer and consider the presence of HDAC1 in high-molecular-weight complexes.
• For immunofluorescence/immunocytochemistry, a permeabilization step is essential due to HDAC1’s nuclear localization; co-staining with a nuclear marker is recommended.
• IHC-P may require antigen retrieval optimization; validate staining with positive and negative tissue controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports epigenetic research and diagnostic assay development by supplying high-quality IVD raw materials, including extensively validated antibodies like this HDAC1 polyclonal antibody. Our portfolio encompasses optimized antibody pairs, recombinant proteins, and bulk ancillary reagents, complemented by custom sourcing for rare targets. Partner with us for a streamlined, one-stop solution from concept to clinic.