Anti-Hd3a Rabbit Polyclonal Antibody for ELISA and WB - Q93WI9

Core Product Specifications and Parameters

Biological Background: Hd3a Function and Localization

• Hd3a (HEADING DATE 3A), also known as FT-like protein A, is a member of the phosphatidylethanolamine-binding protein (PEBP) family that functions as a mobile florigen in rice.
• Under short-day conditions, Hd3a acts downstream of Hd1 and Ehd1 to promote the transition from vegetative to reproductive growth by moving from leaves to the shoot apical meristem (SAM).
• Once in the SAM, Hd3a induces expression of MADS-box transcription factors MADS14 and MADS15, initiating floral meristem identity.
• Subcellularly, Hd3a is localized in both the cytoplasm and nucleus.
• At the protein level, Hd3a is expressed in the inner region of the SAM, stem, and leaf blade vascular tissues.
• Keywords associated with Hd3a include developmental protein, differentiation, flowering, and its 3D structure has been determined.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of rice Hd3a; therefore, this polyclonal antibody may recognize full-length Hd3a as well as potential cleavage products containing this epitope.
• For Western blotting, a predicted band around 20 kDa is expected; consider using rice leaf or shoot tissue extracts as positive controls and validate the antibody under reducing conditions.
• In ELISA, perform a standard indirect ELISA to check reactivity with the recombinant immunogen; cross-reactivity with other PEBP family members (e.g., RFT1) should be tested if required.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw-material provider, CamelBio supports diagnostic manufacturers and research institutes with a comprehensive portfolio of validated antibodies, including plant biology targets like flowering regulators. This rabbit polyclonal antibody against rice Hd3a is suitable for detection in ELISA and WB, and we can offer bulk quantities, technical customization, and complementary reagents for your assay development needs.