Anti-Hd1 Polyclonal Antibody for WB and ELISA - Q9FDX8

Core Product Specifications and Parameters

Biological Background: Zinc finger protein HD1 (Hd1) Function and Localization

• Encoded by the HD1 (HEADING DATE 1) gene in Oryza sativa, Hd1 is a zinc finger transcription factor also known as OsHd1 or SE1.
• Functions as a key regulator of photoperiod-dependent flowering: promotes flowering under short-day conditions and suppresses flowering under long-day conditions.
• Negatively regulates the floral activator HEADING DATE 3a (HD3A) under long days, while positively regulating it under short days.
• Localizes to the nucleus, consistent with its role in transcriptional regulation.
• Post-translational modifications include phosphorylation (phosphoprotein), potentially modulating its activity.
• Classified under developmental protein, DNA-binding, and flowering-related keywords.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-320) of Hd1, which may provide specificity; consider confirming the antibody's reactivity in your target sample by positive control.
• For Western blot, the predicted molecular weight is 43 kDa; ensure proper extraction of nuclear proteins and use appropriate loading controls.
• For ELISA, optimize coating antigen and antibody dilutions under standard indirect ELISA conditions using recombinant Hd1 protein.
• Validate the antibody's performance in different Oryza sativa cultivars or photoperiod conditions, as Hd1 expression may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and plant biology researchers with streamlined access to high-quality IVD raw materials. This anti-Hd1 polyclonal antibody supports flowering pathway studies and crop development research. Beyond individual antibodies, we offer validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets—helping you bridge the gap from concept to clinic efficiently.