Anti-HCRTR2 Polyclonal Antibody for WB, ELISA - O43614

Core Product Specifications and Parameters

Biological Background: Orexin Receptor Type 2 (HCRTR2) Function and Localization

• HCRTR2, also known as orexin receptor type 2 or hypocretin receptor type 2 (OX2R), is a G protein-coupled receptor (GPCR) belonging to the orexin receptor family.
• It binds the neuropeptides orexin-A and orexin-B, which are derived from the prepro-orexin precursor (PubMed:26950369, PubMed:33547286, PubMed:35614071). Related references: PMID:26950369, PMID:33547286, PMID:35614071
• After binding, it can activate both G(q)/11 and G(i)/o heterotrimeric G proteins, triggering distinct downstream signaling cascades (PubMed:35614071). Related references: PMID:35614071
• It plays a critical role in regulating the sleep-wake cycle (By similarity) and contributes to central glucose homeostasis (By similarity).
• The receptor localizes to the cell membrane and contains typical GPCR structural features, including seven transmembrane helices, disulfide bonds, and glycosylation sites.
• Keywords associated with HCRTR2 include cell membrane, disulfide bond, G protein-coupled receptor, glycoprotein, receptor, transducer, and transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 380–444 near the C-terminus, which may provide good epitope accessibility. Consider using denaturing conditions for Western blot to expose linear epitopes.
• For Western blot, a band around 51 kDa is expected based on the predicted molecular weight. Validate using a suitable positive control, such as brain tissue lysate from human or mouse.
• Cross-reactivity is reported for human and mouse; sequence homology should be checked for other species if needed.
• ELISA can be employed for antibody titer determination or to screen for HCRTR2 expression in samples. Optimize coating and detection conditions experimentally.
• As a polyclonal antibody, lot-to-lot variability may occur. Include appropriate controls and standardized protocols in all assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive sourcing solution for IVD raw materials, connecting diagnostic manufacturers and researchers with high-quality reagents like this anti-HCRTR2 polyclonal antibody, which targets a GPCR implicated in sleep and metabolic regulation. Through our streamlined supply chain, we provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and even rare target raw-material sourcing—all to support your assay development from concept to clinic. Let CamelBio be your procurement partner in advancing neuroscience and metabolic research.