Anti-HCRTR1 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - O43613

Core Product Specifications and Parameters

Biological Background: HCRTR1 Function and Localization

• G protein-coupled receptor that binds the neuropeptide orexin-A with high affinity and orexin-B with lower affinity, derived from prepro-orexin. Related references: PMID:9491897, PMID:32669442
• Orexin-A binding activates the G(q)-protein-coupled pathway, which stimulates the phosphatidylinositol-calcium second messenger system. Related references: PMID:32669442
• Orexin-A stimulation promotes beta-arrestin recruitment, leading to receptor internalization. Related references: PMID:15683363, PMID:32669442
• Plays a significant role in the regulation of food intake (by similarity).
• Localized at the cell membrane with seven transmembrane domains characteristic of transmembrane receptors.
• Contains structural features such as disulfide bonds and glycosylation sites, as noted in UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from amino acids 150-250; consider validating specificity using peptide blocking experiments in the relevant sample system.
• For western blot, the expected band is ~48 kDa; use appropriate gel percentage and transfer conditions for this molecular weight.
• In immunofluorescence, anticipate membrane localization staining; co-localize with membrane markers for confirmation.
• Cross-reactivity with mouse and rat is noted; validate in neural tissues such as hypothalamus where orexin pathways are active.
• For ELISA, optimize antibody titer and consider using synthetic orexin-A peptide as a positive control to assess binding affinity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes a comprehensive one-stop sourcing solution for IVD raw materials, technical services, and consulting—from concept to clinic. For neuroscience and metabolic disease research, CamelBio provides validated antibody pairs, optimized polyclonal antibodies like anti-HCRTR1, and bulk ancillary reagents, as well as rare target raw-material sourcing for orexin receptors. Partner with CamelBio to accelerate your diagnostic development with reliable, high-quality raw materials and end-to-end support.