Anti-HCRT Rabbit Polyclonal Antibody for WB, ELISA - O43612

Core Product Specifications and Parameters

Biological Background: HCRT Function and Localization

• HCRT encodes the precursor of orexin neuropeptides, also known as hypocretin, involved in the regulation of feeding behavior and sleep-wake cycles.
• The prepro-orexin precursor is proteolytically processed to produce two active neuropeptides: orexin-A and orexin-B, which bind with high affinity to their respective receptors HCRTR1 and HCRTR2.
• Orexin signaling is implicated in broader homeostatic functions including energy metabolism, autonomic regulation, hormonal balance, and fluid homeostasis.
• HCRT is predominantly expressed in the subthalamic nucleus, with undetectable levels in other tested brain regions and peripheral tissues (heart, placenta, lung, liver, skeletal muscle, kidney, pancreas).
• Subcellularly, the precursor localizes to the rough endoplasmic reticulum and is secreted via cytoplasmic vesicles at synaptic terminals.
• Key post-translational modifications include amidation, disulfide bond formation, and N-terminal pyroglutamic acid formation, which are critical for neuropeptide activity.
• Clinically, HCRT dysfunction is associated with disease variants linked to narcolepsy and other sleep disorders (UniProt keyword: Disease variant).

Experimental Guidance and Technical Tips

• For Western blot detection, consider the predicted molecular weight of 13 kDa; validate the antibody in your sample system using appropriate positive controls from brain tissue (subthalamic nucleus) or recombinant protein.
• Due to restricted HCRT expression, ensure sample enrichment from the central nervous system; tissue from heart, placenta, liver, or kidney may serve as negative controls based on reported undetectable levels.
• In ELISA applications, the antibody can be explored as a capture or detection reagent; optimize coating concentration and blocking conditions empirically.
• Cross-reactivity with mouse and rat orthologs has been reported; however, sequence alignment of the immunogen region across species is recommended to confirm specificity.
• Consider pre-absorption or peptide competition assays to confirm specificity if non-specific bands appear in Western blot.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a seamless sourcing experience for IVD raw materials, catering to diagnostic manufacturers and research laboratories worldwide. For your orexin/hypocretin-related studies, we supply validated polyclonal antibodies, antibody pairs, and bulk ancillary reagents to support immunoassay development. Whether you need rare target raw-material sourcing or comprehensive technical consultation from concept to clinic, CamelBio is your trusted partner in advancing neuroscience and metabolic research.