Anti-HCoV-OC43 Spike S2 Rabbit Polyclonal Antibody for WB, ELISA - P36334

Core Product Specifications and Parameters

Biological Background: Spike glycoprotein Function and Localization

• Spike glycoprotein (S), also designated E2 or peplomer protein, is a class I viral fusion protein decorating the surface of HCoV-OC43 virions.
• The S1 subunit mediates initial attachment to host cells by binding to sialic acid-containing receptors, a key determinant of viral tropism.
• Following receptor engagement and virus endocytosis, the S2 subunit undergoes dramatic conformational changes; its heptad repeat regions form a trimer-of-hairpins that drives fusion of viral and host endosomal membranes.
• Spike is synthesized as a precursor and proteolytically cleaved into S1 and S2; the internal fusion peptide within S2 is unmasked after cleavage, enabling membrane fusion.
• During viral assembly, the protein localizes to the virion membrane, the host cell membrane, and the endoplasmic reticulum–Golgi intermediate compartment (ERGIC) membrane.
• Post-translational modifications include N-linked glycosylation, disulfide bonds stabilizing the prefusion structure, and palmitoylation anchoring the protein in the lipid bilayer.
• As a key virulence factor mediating both host cell attachment and entry, spike is a major target for neutralizing antibodies and diagnostic reagent development.

Experimental Guidance and Technical Tips

• The immunogen peptide lies within the S2 subunit (aa 782–881); therefore, the antibody is expected to recognize both full-length Spike (~150 kDa) and the S2 cleavage product under reducing conditions.
• For WB, consider using HCoV-OC43-infected cell lysate as a positive control; glycosylation may cause the band to migrate at a higher apparent molecular weight.
• Due to the polyclonal nature, the antibody may provide broad epitope coverage in ELISA; however, cross-reactivity with other coronaviruses should be assessed empirically.
• As a transmembrane glycoprotein, Spike requires appropriate detergents for extraction; avoid prolonged boiling of samples to prevent aggregation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research institutes to deliver a comprehensive supply of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For HCoV-OC43 spike research and assay development, we offer direct access to this anti-Spike S2 antibody and can source matched antigens and detection tools. Our one-stop service supports your workflow from concept to clinic, streamlining procurement and accelerating project timelines.