Anti-HCoV-OC43 Nucleoprotein Polyclonal Antibody for WB, ELISA - P33469

Core Product Specifications and Parameters

Biological Background: Nucleoprotein Function and Localization

• The Human coronavirus OC43 Nucleoprotein (N protein), also known as Nucleocapsid protein (NC), is a structural protein essential for viral genome packaging.
• Packages the positive-strand viral RNA genome into a helical ribonucleocapsid (RNP) and plays a key role in virion assembly via interactions with the viral genome and membrane protein M.
• Enhances subgenomic viral RNA transcription and viral replication efficiency.
• Attenuates host stress granule formation by reducing G3BP1 access to host mRNAs under stress conditions. Related references: PMID:36534661
• Subcellular localization includes virion, host endoplasmic reticulum-Golgi intermediate compartment, and host Golgi apparatus.
• Post-translational modifications and functional features include ADP-ribosylation, phosphorylation, and RNA-binding; associated with transcription regulation and viral nucleoprotein activity.

Experimental Guidance and Technical Tips

• For Western blot (WB), expect a band at approximately 49 kDa; validate with positive control lysates from HCoV-OC43 infected cells or recombinant N protein.
• For ELISA, this polyclonal antibody can be used as a capture or detection reagent; optimize coating and detection conditions empirically.
• As a rabbit polyclonal antibody, performance may vary between lots; consider performing lot-specific validation in your assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering diagnostic manufacturers, labs, and research institutes one-stop access to supplies, technical services, and consulting from concept to clinic. For coronavirus targets like HCoV-OC43 Nucleoprotein, we provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Let CamelBio streamline your assay development and supply chain.