Anti-HCoV-NL63 Spike S2 Polyclonal Antibody for WB, ELISA - Q6Q1S2

Core Product Specifications and Parameters

Biological Background: Spike glycoprotein Function and Localization

• Spike glycoprotein (S protein, E2, peplomer protein) is the major envelope protein of HCoV-NL63, mediating viral entry into host cells.
• The S1 subunit binds to the host receptor angiotensin-converting enzyme 2 (ACE2) to initiate infection. Related references: PMID:15897467 PMID:19901337 PMID:29142129
• The S2 subunit is a class I viral fusion protein that undergoes dramatic conformational changes to fuse viral and host membranes.
• The protein transitions through at least three states: pre-fusion native, pre-hairpin intermediate, and post-fusion hairpin, with coiled-coil heptad repeats forming a trimer-of-hairpins structure.
• Located on the virion membrane and in host endoplasmic reticulum-Golgi intermediate compartment membranes.
• Contains disulfide bonds and is heavily glycosylated, typical of viral envelope proteins, and functions in viral attachment and entry.
• Also listed as involved in virulence and host-virus interaction.

Experimental Guidance and Technical Tips

• As a polyclonal antibody generated against a peptide immunogen in the S2 region, it may recognize both native and denatured Spike protein; verify by pilot experiments.
• For Western blotting under reducing conditions, expect a band at approximately 150 kDa; run appropriate molecular weight markers.
• For ELISA applications, consider using as a coating antibody or detection antibody paired with a suitable secondary antibody; validate with HCoV-NL63 antigen.
• Since cross-reactivity is reported only with HCoV-NL63, do not assume detection of other coronaviruses without experimental confirmation.

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