Anti-HCoV-HKU1 Spike S2 Rabbit Polyclonal Antibody for WB, ELISA - Q5MQD0

Core Product Specifications and Parameters

Biological Background: Spike Glycoprotein Function and Localization

• The spike glycoprotein (S protein) of HCoV-HKU1, also known as E2 or peplomer protein, is a key structural component of the viral envelope and exists as a homotrimer on the virion surface.
• It mediates viral attachment to host cells by interacting with the host receptor, initiating the infection process.
• The S protein is a class I viral fusion protein that drives fusion between the viral and host cell membranes through a series of conformational changes, including the formation of a trimer-of-hairpins structure in the S2 subunit.
• After viral endocytosis, the S2 subunit acts as a fusion peptide, unmasked upon proteolytic cleavage, to facilitate virus entry into the host cytoplasm.
• Subcellular localization includes the virion membrane, host endoplasmic reticulum-Golgi intermediate compartment membrane, and host cell membrane, reflecting its synthesis and trafficking pathway.
• The protein is heavily glycosylated and palmitoylated, containing disulfide bonds, coiled-coil regions, and a transmembrane helix, which are critical for its stability and fusion activity.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the S2 region (aa 800-900), which is conserved in HCoV-HKU1; verify specificity in relevant viral strains or infected cell lysates.
• For Western blot, the predicted molecular weight is ~152 kDa; consider using a low-percentage SDS-PAGE gel and wet transfer to nitrocellulose membrane for optimal detection of this large protein.
• ELISA applications may benefit from optimizing coating antigen concentration and using a blocking buffer suitable for viral antigens to minimize background.
• Cross-reactivity is reported for HCoV-HKU1; if testing other coronaviruses, validate cross-reactivity experimentally, as sequence homology may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this HCoV-HKU1 Spike S2 antibody as part of a comprehensive IVD raw-material portfolio, enabling diagnostic manufacturers to develop accurate coronavirus assays. Our supply capabilities extend to validated antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary reagents for bulk procurement, with the flexibility to support rare target sourcing for emerging infectious disease research. Let CamelBio be your reliable partner from concept to clinic.