Anti-HCoV-HKU1 Nucleoprotein Rabbit Polyclonal Antibody for WB, ELISA - Q5MQC6

Core Product Specifications and Parameters

Biological Background: HCoV-HKU1 Nucleoprotein Function and Localization

• The HCoV-HKU1 nucleoprotein (N protein), encoded by the N gene, is a multifunctional structural protein essential for the viral life cycle.
• Its primary role is to package the positive-strand viral RNA genome into a helical ribonucleocapsid (RNP) via direct RNA binding.
• During virion assembly, the N protein interacts with the membrane (M) protein, facilitating the incorporation of the RNP into budding particles.
• Beyond structural roles, it enhances the efficiency of subgenomic viral RNA transcription and contributes to viral replication.
• The protein localizes to the virion, host endoplasmic reticulum-Golgi intermediate compartment (ERGIC), and host Golgi apparatus, reflecting its dynamic trafficking during infection.
• Post-translational modifications, including phosphorylation and ADP-ribosylation, likely regulate its multiple functions in the replication cycle.
• A resolved 3D structure of the N protein provides molecular insights into its RNA-binding domain and oligomerization interfaces.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning residues 100–200 of the N protein (YP_173242.1); the resulting polyclonal antibody may preferentially recognize linear epitopes in this region. Consider using it for denaturing western blot and ELISA applications.
• In western blot, a band of approximately 49 kDa is expected for the full-length N protein. Validate detection in samples expressing HCoV-HKU1 N protein or infected cell lysates.
• For ELISA, the antibody can be employed as a capture or detection reagent; pairing with a validated N protein-specific monoclonal antibody may improve assay sensitivity.
• As a polyclonal antibody, lot-to-lot variation is possible; it is advisable to titrate the antibody for each new batch and include appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to IVD raw materials, covering every stage from concept to clinic. For coronavirus research and assay development, we supply a broad portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can assist with sourcing rare-target raw materials. Whether you are developing N protein-based immunoassays or need consistent supply for production, CamelBio is your trusted partner for one-stop IVD raw material sourcing.