Anti-HCoV-229E Spike S2 Rabbit Monoclonal Antibody for WB and ELISA - P15423

Core Product Specifications and Parameters

Biological Background: Spike Glycoprotein Function and Localization

• The spike (S) glycoprotein of human coronavirus 229E mediates viral entry into host cells.
• The S protein is cleaved into S1 and S2 subunits; S1 binds to the host receptor aminopeptidase N (ANPEP), while S2 mediates fusion of the viral and cellular membranes.
• The S2 subunit is a class I viral fusion protein containing conserved heptad repeat regions that form a coiled-coil structure.
• Upon receptor binding, S2 undergoes conformational changes, transitioning from a pre-fusion to a post-fusion state via a pre-hairpin intermediate, ultimately forming a trimer-of-hairpins that drives membrane fusion.
• The S protein localizes to the virion membrane and the host endoplasmic reticulum–Golgi intermediate compartment (ERGIC) membrane during viral assembly.
• It is a glycoprotein with roles in viral attachment, entry, and virulence.

Experimental Guidance and Technical Tips

• This antibody was raised against recombinant S2 protein and is recommended for Western blot and ELISA applications.
• The S2 subunit is relatively conserved among coronaviruses; cross-reactivity with other HCoV strains should be validated experimentally.
• For WB, a protein band around 129 kDa is expected; ensure proper denaturing conditions to detect the full-length or cleaved S2 fragment.
• In ELISA, consider using the antibody to capture or detect S2 in viral lysates or recombinant antigen preparations.
• Validate the antibody in the relevant biological context, such as HCoV-229E-infected cell lines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw material sourcing, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For HCoV-229E research and diagnostic development, we supply high-quality spike protein antibodies, including this anti-S2 monoclonal antibody, to support viral entry and fusion studies. Our one-stop service bridges the gap between concept and clinic, ensuring reliable supply of critical raw materials.