Anti-HCoV-229E Spike S2 Polyclonal Antibody for WB, ELISA - P15423

Core Product Specifications and Parameters

Biological Background: Spike glycoprotein Function and Localization

• Spike glycoprotein (S protein), also known as E2 or peplomer protein, is encoded by the S gene of HCoV-229E.
• The S protein mediates viral entry by attaching to the host receptor aminopeptidase N (ANPEP) via its S1 subunit, followed by S2-mediated membrane fusion.
• S2 is a class I viral fusion protein that undergoes conformational changes from pre-fusion to post-fusion states, forming a trimer-of-hairpins structure to fuse viral and host membranes.
• Contains heptad repeat regions that form coiled-coil structures essential for fusion, positioning the fusion peptide near the C-terminal region of the ectodomain.
• Spike glycoprotein is found in the virion membrane and the host endoplasmic reticulum–Golgi intermediate compartment (ERGIC) membrane during viral assembly.
• Glycoprotein, host-virus interaction, viral envelope protein, virulence factor, and virus entry into host cell are key functional keywords.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a conserved linear epitope within the S2 region (aa 1074–1173). Consider using this antibody to detect both precursor and cleaved S2 fragments in infected cell lysates or purified virions.
• For Western blot, the predicted full-length spike molecular weight is 129 kDa; proteolytic processing may produce S2 fragments of varying sizes. Validate specificity with appropriate controls (e.g., uninfected cells).
• This polyclonal antibody is suitable for ELISA. Consider antigen-coating conditions (native or denatured) to optimize signal. Cross-reactivity is confirmed with HCoV-229E; if testing other coronaviruses, validate independently.

CamelBio: Your One-Stop Sourcing Bridge

Sourcing reliable antibodies for human coronavirus research is critical for diagnostic development. CamelBio offers one-stop access to validated IVD raw materials, including this HCoV-229E spike polyclonal antibody, along with optimized monoclonal antibodies, antigen proteins, and ancillary reagents. For rare targets like coronavirus spike proteins, CamelBio’s global sourcing network ensures consistent supply to support your assay development and manufacturing needs, helping you transition smoothly from concept to clinical application.