Anti-HCoV-229E Spike S1 Polyclonal Antibody for WB, ELISA - P15423

Core Product Specifications and Parameters

Biological Background: HCoV-229E Spike S1 Function and Localization

• The Spike (S) glycoprotein is a structural protein of Human coronavirus 229E, essential for viral entry. The protein is synthesized as a precursor and cleaved into S1 and S2 subunits.
• S1 subunit mediates initial attachment to the host cell by binding to the receptor aminopeptidase N (ANPEP), which determines cell tropism and host range.
• S2 subunit is a class I viral fusion protein containing heptad repeat regions. Following receptor binding, S2 undergoes extensive conformational changes to form a trimer-of-hairpins structure, driving fusion between the viral envelope and host cell membrane.
• During intracellular transport, the Spike protein localizes to the virion membrane and the host endoplasmic reticulum-Golgi intermediate compartment (ERGIC) membrane, where viral assembly occurs.
• The protein is heavily glycosylated, which may contribute to immune evasion and correct folding.
• No antimicrobial or tissue-specific functional roles have been documented; its primary function is mediating virus entry.

Experimental Guidance and Technical Tips

• The antibody is raised against a synthetic peptide within the S1 region (aa 1074–1173). Confirm sequence conservation if using with viral isolates other than the reference strain.
• Predicted molecular weight of full-length Spike is approximately 129 kDa. For Western blot, ensure sufficient transfer and use appropriate molecular weight markers.
• For ELISA, consider pairing with a capture antibody targeting a distinct epitope to avoid competition.
• This antibody is expected to specifically recognize HCoV-229E Spike; cross-reactivity with other human coronaviruses (e.g., OC43, NL63) has not been tested and should be validated if used in multiplex assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio bridges the gap between raw material sourcing and IVD product development. As a specialized provider for diagnostics, CamelBio offers validated antibody reagents—like this anti-HCoV-229E Spike S1 polyclonal antibody—to support infectious disease research and assay design. In addition to individual antibodies, CamelBio can supply matched antibody pairs, recombinantly expressed proteins, and bulk ancillary reagents, streamlining your path from concept to clinic.