Anti-HCoV-229E Spike S1 Monoclonal Antibody for WB, ELISA - P15423

Core Product Specifications and Parameters

Biological Background: Spike Glycoprotein Function and Localization

• The spike (S) glycoprotein of human coronavirus 229E (HCoV-229E) is a class I viral fusion protein essential for viral entry into host cells.
• It is synthesized as a precursor and proteolytically cleaved into S1 and S2 subunits; the S1 region contains the receptor-binding domain (RBD) that attaches to the host receptor aminopeptidase N (ANPEP).
• Receptor engagement triggers conformational rearrangements that expose the S2 fusion peptide, initiating membrane fusion between the viral envelope and host cell membrane.
• The S2 subunit features heptad repeat regions that assemble into a characteristic trimer-of-hairpins structure, driving apposition and fusion of the viral and target membranes.
• Subcellularly, the spike protein localizes to the virion membrane and the host endoplasmic reticulum–Golgi intermediate compartment (ERGIC) during virion assembly.
• Key functional and structural features include a signal peptide, transmembrane helix, coiled-coil domains, extensive glycosylation, and roles in virulence, host-virus interaction, and viral attachment.

Experimental Guidance and Technical Tips

• For Western blot analysis, consider using reducing conditions; the full-length spike protein migrates at approximately 129 kDa, while the S1 cleavage product may appear as a smaller band. Validate antibody reactivity in HCoV-229E infected cell lysates or spike‑expressing cells.
• In ELISA applications, the antibody can serve as a capture or detection reagent for HCoV-229E spike S1 protein; titrate the working concentration to optimize signal-to-noise ratio.
• The antibody is specific for HCoV-229E and does not cross‑react with phylogenetically distinct human coronaviruses; confirm selectivity in multipathogen panels if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw‑material sourcing, supporting every stage from concept to clinic. This HCoV-229E Spike S1 monoclonal antibody empowers coronavirus research and diagnostic development. We provide validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and rare‑target raw‑material sourcing to accelerate your assay development with confidence.