Anti-HCLS1 Polyclonal Antibody for WB, IHC-P, ELISA - P14317

Core Product Specifications and Parameters

Biological Background: HCLS1 Function and Localization

• HCLS1 (Hematopoietic lineage cell-specific protein), also known as HS1, p75, LckBP1, and CTTNL, is a substrate of antigen receptor-coupled tyrosine kinases and plays a critical role in antigen receptor signaling for clonal expansion and deletion in lymphoid cells.
• The protein is expressed exclusively in hematopoietic cells and tissues, highlighting its importance in immune cell biology.
• HCLS1 localizes to the membrane, cytoplasm, and mitochondrion, suggesting multi-compartmental functions in signal transduction.
• It may also regulate gene expression, though the precise mechanism requires further investigation.
• The protein contains an SH3 domain and undergoes post-translational modifications including phosphorylation and acetylation, as identified by proteomics and direct protein sequencing.
• Alternative splicing produces multiple isoforms, potentially diversifying its functional roles.
• The predicted molecular weight is approximately 54 kDa (UniProt: 54014 Da), consistent with experimental observations.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (amino acids 1-280); consider that this sequence may be conserved across isoforms and between human and mouse, as reflected in the cross-reactivity.
• For Western blotting (WB), expect a band near 54 kDa and validate the antibody using lysates from hematopoietic cell lines such as Jurkat, human PBMCs, or mouse splenocytes.
• In immunohistochemistry (IHC-P), optimize antigen retrieval conditions carefully, as HCLS1 expression is restricted to infiltrating hematopoietic cells within tissues.
• For ELISA applications, pair with an appropriate detection antibody and confirm reactivity using recombinant HCLS1 protein or cell-based materials.
• Because HCLS1 is a phosphoprotein, include phosphatase inhibitors during sample preparation to preserve phosphorylation states if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop supply partner for IVD raw materials, supporting development from concept to clinic. This anti-HCLS1 polyclonal antibody facilitates research into hematopoietic lineage and antigen receptor signaling. We offer validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom rare-target raw-material sourcing to meet your assay development demands.