Anti-HCK Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P08631

Core Product Specifications and Parameters

Biological Background: HCK Function and Localization

• Tyrosine-protein kinase HCK is a non-receptor kinase predominantly expressed in hematopoietic cells, particularly myeloid and B-lymphoid lineages; alternative splicing and initiation produce isoforms p59Hck and p61Hck.
• Acts as a key signal transducer downstream of various receptors (Fc receptors, cytokine receptors, integrins) to regulate innate immune responses, including phagocytosis, degranulation, respiratory burst, cell adhesion, migration, and survival.
• Mediates phosphorylation of multiple substrates such as CBL, VAV1, STAT5B, and WAS, thereby controlling actin cytoskeleton reorganization, podosome formation, and cell protrusion.
• Localizes to multiple subcellular compartments including plasma membrane, caveolae, focal adhesions, podosomes, cytoplasm, lysosomes, secretory vesicles, Golgi, and nucleus, reflecting its dynamic role in signal transduction.
• Highly expressed in granulocytes, and detected in monocytes and neutrophils; also found in tonsil tissue.
• Contains SH2 and SH3 domains for protein-protein interactions and an ATP-binding kinase domain; subject to myristoylation and palmitoylation for membrane targeting.
• Identified as a proto-oncogene and reference proteome member; also undergoes ubiquitin-like conjugation.

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against the N-terminal region (aa 1-220) of human HCK, which may detect both p59 and p61 isoforms; consider isoform-specific validation if needed.
• For WB, a band around 60 kDa is expected; post-translational modifications may cause minor electrophoretic shifts.
• Recommended applications include WB, IF/ICC, and ELISA; optimize dilutions and appropriate controls for each assay system.
• Reported cross-reactivity is for human; for other species, check sequence homology in the immunogen region before use.

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