Anti-hCG beta Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P0DN86

Core Product Specifications and Parameters

Biological Background: hCG beta Function and Localization

• hCG beta (CGB) is the beta subunit of human chorionic gonadotropin, a glycoprotein hormone composed of non-covalently linked alpha and beta subunits; the alpha subunit is shared with LH, FSH, and TSH, while the beta subunit determines receptor specificity and biological activity.
• Encoded by the CGB gene family (CGB3, CGB5, CGB8), the protein is primarily expressed in the placenta throughout pregnancy where it plays a critical role in maternal adaptation and pregnancy maintenance.
• hCG stimulates ovarian steroidogenesis, which is essential for sustaining pregnancy; the CGB subunit specifically activates the LH/CG receptor.
• Upon secretion, the hormone circulates as a heterodimer; post-translational modifications include N- and O-linked glycosylation and multiple disulfide bonds that stabilize its tertiary structure.
• Keywords associated with the protein include hormone, glycoprotein, secreted, disulfide bond, signal peptide, and pharmaceutical, highlighting its functional and therapeutic relevance.
• Alternative splicing generates multiple transcript variants, and the protein is further characterized by a cystine-knot structural motif.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 20-165 of human CGB5, covering the mature beta subunit; this design may recognize both full-length and processed forms of hCG beta.
• For ELISA setups, consider pairing this antibody with a suitable anti-alpha subunit antibody to capture intact hCG heterodimers.
• In immunohistochemistry and immunofluorescence experiments, note that hCG is secreted; signal may localize to syncytiotrophoblast cells in placental tissue sections.
• The antibody is validated for human reactivity; cross-reactivity with other species has not been assessed and should be tested if non-human samples are used.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with integrated access to validated IVD antibodies, including this anti-hCG beta monoclonal antibody, to support the development of pregnancy and fertility assays. We offer one-stop sourcing for antibody pairs, bulk ancillary reagents, and customized rare-target reagent procurement to streamline your transition from concept to clinic.