Anti-HCFC1 Rabbit Polyclonal Antibody for WB, ELISA - P51610

Core Product Specifications and Parameters

Biological Background: HCFC1 Function and Localization

• HCFC1 is a transcriptional coregulator that serves as a scaffold protein, mediating interactions between transcription factors like THAP11 and ZNF143 and coregulators (PubMed:26416877). Related references: PMID:26416877
• It is critical for cell cycle progression, with functional roles in G1/S transition, supported by studies using mutants and knockdown models (PubMed:10629049, PubMed:10779346, PubMed:15190068). Related references: PMID:10629049 PMID:10779346 PMID:15190068
• HCFC1 tethers the Set1/Ash2 histone H3K4 methyltransferase and Sin3 histone deacetylase complexes, integrating transcriptional activation and repression (PubMed:12670868). Related references: PMID:12670868
• It functions as a coactivator for EGR2 and GABP2 and represses ZBTB17-mediated activation of the p15(INK4b) promoter, inhibiting p300 recruitment (PubMed:12244100, PubMed:14532282). Related references: PMID:12244100 PMID:14532282
• As part of the NSL complex, HCFC1 likely contributes to nucleosomal histone H4 acetylation on specific lysine residues (PubMed:20018852). Related references: PMID:20018852
• HCFC1 recruits KMT2E/MLL5 to E2F1-responsive promoters, promoting transcriptional activation and facilitating G1 to S phase transition (PubMed:23629655). Related references: PMID:23629655
• Subcellular localization includes both the cytoplasm and nucleus, consistent with its diverse regulatory roles.
• The protein is widely expressed, with particularly high levels in fetal tissues and adult kidney, and is involved in host-virus interactions, notably with herpes simplex virus VP16.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 250 amino acids of human HCFC1. When using this polyclonal antibody, multiple epitope recognition may yield robust signal, but batch-to-batch consistency should be confirmed for critical assays.
• For western blotting, the predicted molecular weight is ~209 kDa. Use a low-percentage SDS-PAGE gel (e.g., 6-8%) and ensure efficient transfer via wet tank blotting with extended transfer time. Including a high-molecular-weight marker is recommended.
• Due to the high molecular weight, consider using fresh protein lysates with protease inhibitors to prevent degradation, and verify expression in your target tissue; kidney and fetal tissue lysates may serve as positive controls.
• The antibody shows cross-reactivity with mouse and rat samples by supplier data. However, sequence alignment of the immunogen region across species is advised to ensure expected reactivity. Perform validation with appropriate controls.
• For ELISA applications, optimize coating antigen and antibody concentration; consider potential issues of high background due to polyclonal nature.

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