Anti-HCAR1 Rabbit Polyclonal Antibody for WB and ELISA - Q9BXC0

Core Product Specifications and Parameters

Biological Background: HCAR1 Function and Localization

• Hydroxycarboxylic acid receptor 1 (HCAR1), also known as GPR81 or HCA1, is a member of the G protein-coupled receptor (GPCR) family.
• HCAR1 functions as a receptor for L-lactate and mediates its anti-lipolytic effect through a G(i)-protein-mediated signaling pathway.
• The receptor is highly expressed in brown and white adipose tissues, with lower levels detected in liver, kidney, skeletal muscle, brain, and pituitary, but not in specific cortical or hippocampal regions.
• HCAR1 is localized to the cell membrane, consistent with its role as a transmembrane receptor.
• The protein contains structural features typical of GPCRs, including seven transmembrane helices, and is post-translationally modified by glycosylation and disulfide bond formation.
• HCAR1 has a calculated molecular weight of approximately 39 kDa and is part of the reference proteome with an available 3D structure.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to the C-terminal region (amino acids 250-346) of human HCAR1, which is likely presented in a native-like conformation; consider this when designing epitope competition assays.
• For Western blotting, a band around 39 kDa is expected, though the observed molecular weight may shift slightly due to glycosylation; validate with a suitable positive control such as adipose tissue lysates.
• When performing ELISA, optimize coating conditions and antibody concentrations for your specific assay format; cross-reactivity with mouse and rat HCAR1 allows for translational studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. With this anti-HCAR1 antibody, researchers can advance their work on metabolic signaling and GPCR biology, while CamelBio ensures reliable, one-stop access to the critical reagents and technical support needed from concept to clinic.