Anti-HBB Polyclonal Antibody for WB, ELISA - P68871

Core Product Specifications and Parameters

Biological Background: Hemoglobin subunit beta Function and Localization

• Beta-globin is a major component of adult hemoglobin A (HbA), a tetramer of two alpha and two beta chains, essential for oxygen transport from lungs to peripheral tissues.
• The protein binds heme and iron, enabling reversible oxygen binding; mutations in HBB cause hemoglobinopathies such as sickle cell anemia and beta-thalassemia.
• A processed peptide, LVV-hemorphin-7, derived from beta-globin, potentiates bradykinin activity, contributing to blood pressure regulation.
• Beta-globin acts as an endogenous inhibitor of enkephalin-degrading enzymes like DPP3 and as a selective antagonist of the P2RX3 receptor, implicating it in pain and inflammation modulation.
• Expression is restricted to red blood cells, consistent with its specialized role in erythrocyte oxygen carriage.
• Post-translational modifications include acetylation, glycation, phosphorylation, and S-nitrosylation, affecting protein function and stability.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 47–147 of human HBB (central region); consider that the antibody may recognize both native and denatured forms, but verification in the relevant sample system is advised.
• For Western blot, a band at approximately 16 kDa is expected; include appropriate positive controls such as human or rat red blood cell lysates, and optimize blocking and antibody dilution conditions.
• For ELISA, the polyclonal nature may provide robust capture or detection performance; validate pairing with a matched antibody clone if used in sandwich format.
• Cross-reactivity with rat is reported, but homology with other species should be assessed in silico (e.g., alignment of immunogen sequence) before testing on non-validated species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and researchers with a comprehensive portfolio of IVD raw materials, including validated polyclonal antibodies like this anti‑HBB reagent for hemoglobin and anemia research. Our one‑stop platform offers optimized antibody pairs, bulk ancillary reagents, and rare‑target sourcing to streamline your assay development. Partner with CamelBio to bridge your concept to clinical application efficiently.