Anti-HBA2 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P69905

Core Product Specifications and Parameters

Biological Background: HBA2 Function and Localization

• Encoded by the HBA1 and HBA2 genes, hemoglobin subunit alpha is a major oxygen-binding heme protein essential for transporting oxygen from the lungs to peripheral tissues.
• Hemopressin, a peptide derived from the alpha-globin chain, acts as an antagonist of the cannabinoid receptor CNR1, effectively blocking downstream signaling. Related references: PMID:18077343
• Subunit alpha combines with beta globin to form hemoglobin A, the predominant adult hemoglobin, with critical roles in gas exchange and pH buffering of red blood cells.
• Predominantly expressed in red blood cells, with high abundance in the cytosol as a stable hemoglobin tetramer.
• Post-translational modifications include acetylation, glycation, and phosphorylation; the protein binds heme and iron as a metalloprotein essential for oxygen coordination.
• Mutations in alpha-globin can lead to hereditary hemolytic anemias such as hemoglobin H disease and alpha-thalassemia, underscoring its clinical significance in hematological disorders.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band around 15 kDa under reducing conditions in human, mouse, or rat erythroid cell lysates (e.g., K562 cells) or red blood cell extracts.
• In immunofluorescence/paraformaldehyde-fixed samples, optimize permeabilization steps to detect cytosolic hemoglobin; validate in relevant cellular systems such as erythroleukemia cell lines.
• For ELISA, consider pairing this polyclonal antibody as a capture or detection reagent; confirm optimal working concentrations through titration in your assay buffer.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers with one-stop access to validated IVD raw materials, including this anti-HBA2 polyclonal antibody for hemoglobin research. We offer bulk sourcing of optimized antibody pairs, recombinant proteins, and ancillary reagents, along with expertise in custom antibody development for rare or challenging targets. Partner with us to streamline your assay development from concept to clinic.