Anti-HB-EGF Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q99075

Core Product Specifications and Parameters

Biological Background: HB-EGF Function and Localization

• HB-EGF, also known as DTR (diphtheria toxin receptor), is a member of the epidermal growth factor (EGF) family. It is synthesized as a type I transmembrane precursor (proHB-EGF) that is subsequently cleaved to release a soluble growth factor containing the EGF-like domain.
• The soluble form of HB-EGF acts as a potent ligand for EGFR, ERBB2, and ERBB4, with higher affinity for EGFR than EGF itself, and functions as a potent mitogen for smooth muscle cells and fibroblasts, but not endothelial cells.
• HB-EGF is essential for normal cardiac valve formation and heart function, and it promotes smooth muscle cell proliferation, highlighting its role in cardiovascular development and disease.
• Beyond its growth factor activity, the transmembrane proHB-EGF serves as the receptor for diphtheria toxin, mediating its entry into cells.
• Subcellularly, proHB-EGF localizes to the cell membrane as a single-pass transmembrane protein, while the cleaved soluble form is secreted into the extracellular space, enabling both juxtacrine and paracrine signaling.
• According to UniProt, HB-EGF is characterized by an EGF-like domain, heparin-binding motifs, and multiple disulfide bonds, and undergoes proteolytic processing to generate the mature growth factor.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 20-160 of human HB-EGF, covering a large portion of the extracellular domain. This design targets the soluble growth factor and the precursor, enabling detection of both forms in relevant applications.
• For Western blot, a band around 23 kDa may be observed, consistent with the predicted molecular weight of the full-length precursor. Additional bands corresponding to processed forms may also appear depending on the sample.
• In immunofluorescence/ICC, membrane and/or cytoplasmic staining patterns may be observed, reflecting the precursor and potentially internalized or secreted forms. Appropriate controls and fixation/permeabilization methods should be validated.
• For ELISA, consider pairing this antibody with a matched detection antibody or performing standard titration to optimize sensitivity and specificity for the target analyte.
• Cross-reactivity with mouse and rat HB-EGF is reported; however, experimental conditions should be validated for each species and application, as reactivity may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For researchers studying HB-EGF and related growth factor signaling pathways, we provide streamlined access to high-quality antibodies and support from development to production scale-up. Whether you need rare target reagents or one-stop procurement, CamelBio bridges your research to reliable supply.