Anti-HB-EGF Rabbit Monoclonal Antibody for WB, ELISA - Q99075

Core Product Specifications and Parameters

Biological Background: HB-EGF Function and Localization

• Proheparin-binding EGF-like growth factor (HB-EGF) is synthesized as a transmembrane precursor protein that undergoes proteolytic cleavage to release the soluble growth factor.
• HB-EGF acts as a ligand for the EGF receptor (EGFR), ERBB2, and ERBB4, mediating cellular proliferation, particularly in smooth muscle cells; it is a more potent mitogen for these cells than EGF itself.
• The protein is essential for normal cardiac valve formation and heart function, and it may participate in macrophage-mediated cellular proliferation.
• HB-EGF also functions as the cellular receptor for diphtheria toxin, enabling toxin entry into cells.
• Subcellular localization includes the cell membrane as a transmembrane protein and the extracellular space as the shed soluble form.
• Key structural features include an EGF-like domain, heparin-binding motifs, disulfide bonds, and glycosylation sites.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a sequence within amino acids 50–150 of human HB-EGF, covering a region distinct from the EGF-like domain; users should verify this epitope’s accessibility in their specific assay format.
• For Western blotting, the predicted molecular weight of the pro-form is approximately 23 kDa; a band near this size is expected, though post-translational modifications may cause shifts. Consider using reducing conditions and appropriate loading controls.
• In ELISA applications, the antibody may be used for capture or detection; perform cross-reactivity and specificity testing in the relevant sample matrix (e.g., human serum, tissue lysates).
• Given the reported cross-reactivity with mouse and rat, the antibody can be employed in comparative studies; however, we recommend validating reactivity in the intended species and model system through pilot experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes streamlined access to high-quality IVD raw materials like this HB-EGF rabbit monoclonal antibody. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing capabilities for rare targets. Whether you need consistent supply for kit development or technical support for assay design, CamelBio bridges every stage from concept to clinic.