Anti-HAS3 Polyclonal Antibody for WB, IF/ICC, ELISA - O00219

Core Product Specifications and Parameters

Biological Background: Hyaluronan Synthase 3 (HAS3) Function and Localization

• Hyaluronan synthase 3 (HAS3) is one of three isoenzymes (HAS1, HAS2, HAS3) responsible for hyaluronan synthesis, a key component of extracellular matrices.
• HAS3 catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer, which is critical for tissue architecture and cell behavior regulation.
• It is an integral membrane protein localized to the cell membrane, Golgi apparatus membrane, and trans-Golgi network membrane, as well as early endosomes.
• The protein is post-translationally modified by glycosylation and contains transmembrane helices, typical of glycosyltransferase enzymes.
• Alternative splicing generates multiple isoforms, expanding the functional diversity of HAS3 in hyaluronan synthesis.
• Hyaluronan produced by HAS3 contributes to cell adhesion, migration, and differentiation processes, influencing development and disease.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a conserved intracellular domain (amino acids 67-281) of HAS3, which may provide broad species cross-reactivity as observed.
• For Western blot, validate the antibody in lysates of human, mouse, or rat cells expressing HAS3; expected band near 63 kDa, but glycosylation may cause slight shifts.
• In immunofluorescence/ICC, expect membrane and Golgi localization; permeabilization is required for intracellular epitopes.
• For ELISA, use recombinant HAS3 protein or peptide as coating antigen; test linear range empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, providing access to validated antibodies like this anti-HAS3 polyclonal antibody for hyaluronan and extracellular matrix research. Our extensive portfolio includes optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your diagnostic assay development.