Anti-HAS3 Polyclonal Antibody for WB and ELISA - O00219

Core Product Specifications and Parameters

Biological Background: HAS3 Function and Localization

• Hyaluronan synthase 3 (HAS3) is a membrane-bound enzyme that catalyzes the addition of GlcNAc and GlcUA monosaccharides to synthesize hyaluronan, a major component of extracellular matrices.
• It belongs to the HAS family of three isoenzymes (HAS1, HAS2, HAS3) responsible for cellular hyaluronan synthesis, each with distinct tissue distribution and regulatory properties.
• HAS3 localizes to multiple membrane compartments, including the plasma membrane, Golgi apparatus, trans-Golgi network, and early endosomes, reflecting its role in hyaluronan secretion.
• The protein undergoes alternative splicing, generating multiple isoforms that may differ in activity or localization.
• As a glycosyltransferase, HAS3 regulates tissue architecture, cell adhesion, migration, and differentiation through its control of extracellular matrix composition.
• UniProt keywords indicate that HAS3 is a transmembrane helix‑containing glycoprotein, consistent with its membrane topology and glycosylation.

Experimental Guidance and Technical Tips

• The immunogen for this antibody is a recombinant fusion protein covering amino acids 67–281 of human HAS3. Consider sequence alignment to assess conservation across species and isoforms.
• For Western blot (WB), the predicted molecular weight is ~63 kDa but may appear as multiple bands due to glycosylation, isoforms, or cleavage. Validate specificity with appropriate controls and known positive samples.
• When performing ELISA, optimize coating antigen and antibody dilution using a checkerboard titration. Since this is a polyclonal antibody, lot-to-lot consistency should be verified.
• This antibody has shown cross-reactivity with mouse and rat, but users should confirm reactivity in their specific sample types due to potential species-specific differences.
• For immunohistochemistry or immunofluorescence, note that this product is not validated for those applications; if attempting, perform thorough optimization and include appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio enables diagnostic manufacturers, laboratories, and research institutes to access a comprehensive portfolio of IVD raw materials, covering every stage from concept to clinic. For hyaluronan biology and related disease research, CamelBio offers this anti-HAS3 rabbit polyclonal antibody alongside validated monoclonal/polyclonal alternatives, antibody pairs, and bulk ancillary reagents. With expertise in rare target sourcing, we streamline your supply chain—from early-stage assay development through to large-scale production.