Anti-HAS2 Rabbit Polyclonal Antibody for WB, ELISA - Q92819

Core Product Specifications and Parameters

Biological Background: HAS2 Function and Localization

• Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer, a critical step in hyaluronan synthesis. Related references: PMID:20507985 PMID:21228273 PMID:23303191

• Serves essential roles in tissue architecture, cell adhesion, migration, and differentiation through the production of hyaluronan, a major extracellular matrix component. Related references: PMID:20507985 PMID:21228273 PMID:8798477

• One of three isoenzymes (HAS1, HAS2, HAS3) responsible for cellular hyaluronan synthesis; HAS2 is particularly associated with the production of high molecular mass hyaluronan.

• Integral membrane protein localized to the plasma membrane, endoplasmic reticulum membrane, Golgi apparatus membrane, as well as vesicles and lysosomes.

• Identified post-translational modifications include glycosylation, phosphorylation, and ubiquitin-like conjugation (isopeptide bond formation), supported by proteomics identification.

• Expressed in fibroblasts, consistent with its function in extracellular matrix remodeling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a recombinant fragment (amino acids 67-186) of human HAS2; the antibody may recognize both native and denatured forms. Validate in your specific assay conditions.

• For Western blot (WB), consider that HAS2 has a predicted molecular weight of ~64 kDa, though post-translational modifications may result in slight shifts. Optimize electrophoresis and transfer conditions.

• In ELISA applications, test the antibody as a capture or detection reagent after appropriate titration. Cross-reactivity with mouse and rat HAS2 may support translational models.

• Use appropriate positive controls (e.g., fibroblast lysates) and negative controls to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted partner for IVD raw materials, offering a seamless sourcing experience from concept to clinic. This anti-HAS2 antibody supports research in hyaluronan biology and extracellular matrix studies, and can be integrated into broader procurement solutions including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Let us bridge your diagnostic development needs with reliable supply and technical expertise.